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1、ANTICANCERRESEARCH24:1159-1166(2004)AProteinChipSystemforParallelAnalysisofMulti-tumorMarkersanditsApplicationinCancerDetectionZHENGHONGSUN1,XIAOLIFU1,LUZHANG1,XIAOLIYANG1,FEIZHOULIU1andGENGXIHU1,21ResearchCenter,ShanghaiHealthDigitCo.,Ltd.,Shanghai;2In
2、stituteofBiochemistryandCellBiology,ShanghaiInstitutesofBiologicalSciences,ChineseAcademyofSciences,Shanghai,ChinaAbstract.Background:TumormarkersareroutinelymeasuredCancercausesabout7milliondeathsayearworldwide(1),inclinicaloncology.However,theirvaluei
3、ncancerdetectionhasbeingsecondonlytocardiovasculardiseases.Becauseofthebeencontroversiallargelybecausenosingletumormarkerislackofeffectivetherapytocurelatestagecancers,tumorsensitiveandspecificenoughtomeetstrictdiagnosticcriteria.Onescreeningisbyfartheb
4、estwaytodetectcancersearlystrategytoovercometheshortcomingsofsingletumormarkersisenoughtoimprovetheprognosisofcancerpatients.Image-tomeasureacombinationoftumormarkerstoincreasesensitivitybasedtechnologiessuchascomputedtomography(CT)scanandlookfordistinc
5、tpatternstoincreasespecificity.Thisstudyarethemostcommonlyusedmeansforcancerdetection.aimedtodevelopasystemforparalleldetectionoftumormarkersHowever,suchdevicesaretooexpensiveandinconvenientasatoolfortumordetectioninbothcancerpatientsandtooperateinscree
6、ninglargepopulations,especiallyinasymptomaticpopulationsathighrisk.MaterialsandMethods:developingcountries.TheyalsorequiretumorstogrowtoAproteinchipwasfabricatedwithtwelvemonoclonalantibodiesdetectablesize.Examinationoftumormarkershaslongagainstthefollo
7、wingtumormarkersrespectively:CA125,CA15-beensoughtasanothermeansforcancerscreeningbecause3,CA19-9,CA242,CEA,AFP,PSA,free-PSA,HGH,?-HCG,ofthenon-invasivenatureandeaseofusage.NSEandferritin.TumormarkerswerecapturedaftertheproteinTumormarkersrefertosubstan
8、cessecretedbytumorchipwasincubatedwithserumsamples.AsecondaryantibodycellsorotherpartsofthebodyinresponsetothegrowthofconjugatedwithHRPwasusedtodetectthecapturedtumoratumor(2,3).Severaltumormarkers,suchas·-fetoproteinmarkersusing
