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1、Availableonlineat1^n^n^,.sciencedirect.com∞Chinese糯ScienceDirectJournalofNaturalMedicinesChineseJournalofNaturalMedicines2013,l1(5):0519-0524一doi:10.3724/SEJ.1009.2013.00519AntitumoreffectsoftwoextractsfromOxytropisfalcataonhepat0cellularcarcinomainvitroandinvivoYANGGuang—MinglaYANRu2a,WANGZhao
2、—Xian3,ZHANGFang—Fang,PANYang'.CAIBao—ChangKeyLaboratoryofStateAdministrationofTraditionalChineseMedicineforStandardizationofChineseMedicineProcessing&JiangsuKeyLaboratoryofChineseMedicineProcessing,NanjingUniversityofChineseMedicine,Nanjing210023,China:UniversityofMacau,Macau,China,"SchoolofPh
3、armac~ChinaPharmaceuticalUniversity,Nanjing210009,China;LaboratoryofMedicalFungiandPhyto.Biotech,NanjingUniversityofChineseMedicine,Nanjing210023,ChinaAvailableonline20Sept.2013『ABSTRACT1AIMS:ToinvestigatetheantitumoreffectsofextractsfromOxytropisfalcataonhumanhepatocellularcarcinomaSMMC一7721ce
4、llsinvitroandintransplantedmurineH22tumorsinvivo.METHoDS:Cellproliferation.cellcycledistributionandapoptosisinSMMC.7721cellsweredeterminedandtumorgrowthinhibitioninH22tumorswasinvestigated.Cellcycledistributionwasanalyzedbyflowcytometrywithpropidiumiodide(PI)andAnnexinV.FITC,PIdoublestaining.RE
5、SULTS:MTTassayrevealedthatessentialoilandflavonoidsofO.falcata(namedEOOFandFOF、inhibitedproliferationofSMMC一7721cellsinadose—dependentmanner.TheIC50valueofEOOFandFOFwere0.115and0.097mg.mL~,respectively.CellcyclewasarrestedatGphase.a(chǎn)ndinductionofapoptosisoccu~edinSMMC一7721cellswhensubjectedtoFOF
6、.G『rowthinhibitioninH22solidtumorstransplantedmicewassignificantlypronouncedafterbeingtreatedwimF0F.a(chǎn)ndtheinhibitionratiowere56.1%and70.8%attheconcentrationof30and60mg·k2~.CoNCLUSION:TheresultssuggestthatFOFpromotesapoptosisinSMMC一7721cellsandinhibitsH22tumorgrowth,resultinginapotentialantitumo
7、teffectonhepaticcancer.[KEYW0RDS1Antitumor;OxytropisfaIcata;Apoptosis;SMMC一7721:Murinehepatoma22;Proliferation【CLCNumber】R965【Documentcode】A【ArticleID】1672—3651(2013)05—0519—06desperatelyneededinthetreatmentoflivercancerto1IntrOdu