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1、作物學(xué)報(bào)ACTAAGRONOMICASINICA2009,35(6):1038?1043http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2009.01038利用永久F2群體定位小麥株高的QTL*王巖李卓坤田紀(jì)春山東農(nóng)業(yè)大學(xué)/國(guó)家作物生物學(xué)重點(diǎn)實(shí)驗(yàn)室小麥品質(zhì)育種室,山東泰安271018摘要:為研究小麥株高的遺傳機(jī)制,利用DH群體構(gòu)建了一套包含168個(gè)雜交
2、組合的小麥永久F2群體,并于2007年種植于山東泰安和山東聊城。構(gòu)建了一套覆蓋小麥21條染色體的遺傳連鎖圖譜,并利用該圖譜的324個(gè)SSR標(biāo)記對(duì)小麥株高進(jìn)行QTL定位研究,使用基于混合線性模型的QTLNetwork2.0軟件進(jìn)行QTL分析。在永久F2群體中定位了7個(gè)株高QTL,包括4個(gè)加性QTL,1個(gè)顯性QTL,1對(duì)上位性QTL,共解釋株高變異的20%,其中位于4D染色體的qPh4D,具有最大的遺傳效應(yīng),貢獻(xiàn)率為7.5%;位于2D染色體顯性效應(yīng)位點(diǎn)qPh2D,可解釋1.6%的表型變異;位于5B~6D染色體上位效應(yīng)位點(diǎn),
3、可解釋1.7%的表型變異。還發(fā)現(xiàn)加性效應(yīng)、顯性效應(yīng)和上位效應(yīng)對(duì)小麥株高的遺傳起重要作用,并且基因與環(huán)境具有互作效應(yīng)。關(guān)鍵詞:小麥;永久F2群體;株高;數(shù)量性狀位點(diǎn)DetectionofQuantitativeTraitLociforPlantHeightUsinganImmortalizedF2PopulationinWheat*WANGYan,LIZhuo-Kun,andTIANJi-ChunGroupofQualityWheatBreedingofKeyLaboratoryofCropBiology/Shandon
4、gAgriculturalUniversity,Tai’an271018,ChinaAbstract:Tostudythegeneticmechanismofwheatplantheight,asetofdoubledhaploid(DH)lineswereusedtoconstructanimmortalizedF2(IF2)populationcomprising168differentcrosses.TheIF2populationwasevaluatedforplantheightin2007’cropping
5、seasonsinTaianandLiaocheng,Shandongprovince.Linkagemapwasconstructedwith324SSRmarkerscoveringthewholewheatgenome,including284SSR,37ESTsloci,1ISSRlociand2HMW-GSloci,wasconstructed.Thislinkagemapcoveredatotallengthof2485.7cMwithanaveragedistanceof7.67cMbetweenadja
6、centmarkers.QTLanalyseswereperformedusingthesoftwareQTLNetworkversion2.0basedonthemixedlinearmodelatP<0.05.FouradditiveQTLs,1dominanceQTLandpairofepistaticQTLsweredetected,thetotalQTLeffectsdetectedfortheplantheightexplained20%ofthephenotypicvariation.OneQTLqPh4
7、Dforplantheightwasidentifiedonchromosome4D,wasidentifiedonchromosome2D,explaining7.5%ofthephenotypicvariances.DominanceeffectlociqPh2Dwasidentifiedonchromosome2D,explaining1.6%ofthephenotypicvariances;epistaticeffectsoflociwasidentifiedonchromosome5B–6D,explaini
8、ng1.7%ofthephenotypicvariances.Theresultsindicateadditiveeffects,dominanceeffectsandepistaticeffectsareimportantingeneticsofwheatforplantheight,whicharealsosubjectedt
