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1、第44卷第1期淡水漁業(yè)2014年1月Vo1．44No．1FreshwaterFisheriesJan．2014·研究簡報(bào)·泥鰍黏液防生物污損作用研究潘高，劉念，許云，彭俊講，程鈺蓉，郭萍，熊燕飛(武漢理工大學(xué)生物科學(xué)與技術(shù)系，武漢430070)摘要：研究了泥鰍(Misgurnusanguillicaudatus)體表黏液對(duì)微生物結(jié)構(gòu)蛋白與多糖的降解作用和對(duì)微生物生長的影響。采用考馬斯亮藍(lán)法和DNS法檢測(cè)泥鰍黏液與微生物結(jié)構(gòu)大分子混合作用，及黏液上清液中的可溶性蛋白含量和還原糖量。并將泥鰍黏液分別
2、與酵母菌、大腸桿菌、金黃色葡萄球菌、汀內(nèi)船體污損菌FB—a和綠藻混合作用，比較活性黏液和滅活黏液對(duì)它們的影響。結(jié)果顯示，泥鰍黏液對(duì)酵母和大腸桿菌的結(jié)構(gòu)蛋白和多糖沒有顯示出可常規(guī)檢測(cè)到的降解作用；對(duì)大腸桿菌和金黃色葡萄球菌以及江內(nèi)船體污損菌FB—a也沒有顯示出生長抑制或殺菌作用，兩種細(xì)菌和FB—a菌株與泥鰍黏液作用后生長正常，甚至生長更旺盛，且菌細(xì)胞發(fā)生聚集，不易分散；但對(duì)遍生綠藻產(chǎn)生明顯的生長抑制，與泥鰍粘液作用后，綠藻吸附黏液蛋白成分而發(fā)生聚集，貼壁生長明顯減弱。關(guān)鍵詞：泥鰍(Misgurnu
3、sanguillicaudatus)黏液；細(xì)菌；綠藻；生物防污中圖分類號(hào)：$917．4；Q599文獻(xiàn)標(biāo)識(shí)碼：A文章編號(hào)：1000-6907一(2014)01-0098-04StudiesonthebiologicalfoulingpreventionofloachmucusPANGao，LIUNian，XUYun，PENGJun—jiang，CHENGYu-tong，GUOPing，XIONGYah—fei(InstituteofBiologicalSciencesandTechnology，W
4、uhanUniversityofTechnology，Wuhan430070，China)Abstract：Thispaperstudiedtheeffectsofloach(Misgurnusanguillicaudatus)body—surfacemucusonthedegradationofmicrobialstructureproteinandpolysaccharideanditsinfluenceonmicrobialgrowth．Forthesupernateofthemixedl
5、oachmucusandmicrobialsstructuremacromolecules。themethodofcoomassiebrilliantblueWasusedtodetecttheso1ublepro-teincontentandthemethodofDNSwasuseddetectthereducingsugarcontent．ThenmixedloachmucusrespectivelywithE．coil、staphylococcusaureus，shipfoulingbac
6、teriuma(FB—a)andalgae，comparedwiththeinfluenceofthegrowthofactivemucusandinactivatedmucusfortwokindsofpathogenicbacteriaFB—aandalgae．TheresultsshowedthatthemucusobtainedfromtheloachSnaturallifeenvironmentshowednoconventionaldetectabledegradationonthe
7、strueturalDroteinsandpolysaccharidesofyeastandE．coli；loachmucusalsoshowednogrowthinhibitionorbactericidaleffectonE．coli，staphylococcusaureusandshipfoulingbacteriuma(FB—a)，thesetwokindsofpathogenicbacteriaandFB—astrainsshowedaregulargrowtha'ftertreate
8、dwithloachmucus，theyeveRgrewmorevigorouslyandgatheredtogetherSOthatit'sdificulttodisperse；Butforlivinggreenalgae，themucusshowedobviousgrowthinhibition．Greenalgaeadheredtomu—eOHSproteinandgatheredtogether，thenadherentgrowthdecreasedsignificantly．Keywo
