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《氧化樂(lè)果和甲萘威亞致死劑量對(duì)黃褐天幕毛蟲(chóng)2種解毒酶活性的影響.pdf》由會(huì)員上傳分享,免費(fèi)在線閱讀,更多相關(guān)內(nèi)容在應(yīng)用文檔-天天文庫(kù)。
1、第42卷第7期東北林業(yè)大學(xué)學(xué)報(bào)Vo1.42No.72014年7月JOURNALOFNORTHEASTFORESTRYUNIVERSITYJu1.2014氧化樂(lè)果和甲萘威亞致死劑量對(duì)黃褐天幕毛蟲(chóng)2種解毒酶活性的影響)付莉胡春祥(東北林業(yè)大學(xué),哈爾濱,150040)摘要室內(nèi)條件下采用葉片藥膜法分別測(cè)定氧化樂(lè)果和甲萘威對(duì)黃褐天幕毛蟲(chóng)(Malacosom。neustri。test口.cea)3齡幼蟲(chóng)的毒力;采用分光光度法測(cè)定2種藥劑亞致死劑量對(duì)黃褐天幕毛蟲(chóng)的羧酸酯酶(CarE).谷胱甘肽s一轉(zhuǎn)移酶(GSTs)
2、活性的影響。結(jié)果表明:氧化樂(lè)果對(duì)黃褐天幕毛蟲(chóng)的24、48h的Lc,分別為33.975、8.298mg·L~,甲萘威24、48h的LC。分別為23.314、1.863mg·L~。經(jīng)氧化樂(lè)果和甲萘威亞致死劑量處理后,幼蟲(chóng)體內(nèi)的CarE在6~48h內(nèi)均被不同程度抑制,其中處理12h活性降到最低,分別為對(duì)照的0.51、0.36倍:氧化樂(lè)果亞致死劑量對(duì)幼蟲(chóng)體內(nèi)的GSTs表現(xiàn)為抑制一激活一抑制作用,在處理后24h對(duì)該酶的激活作用最強(qiáng),為同期對(duì)照的1.37倍。亞致死劑量甲萘威處理612h內(nèi)幼蟲(chóng)體內(nèi)GSTs被激活,隨
3、后逐漸被抑制。關(guān)鍵詞黃褐天幕毛蟲(chóng):亞致死劑量;羧酸酯酶;谷胱甘肽s一轉(zhuǎn)移酶分類(lèi)號(hào)$763.3EfectofSublethalConcentrationofOmethoateandCarbarylontheActivitiesofTwoDetoxifyingEnzymesinMalacosomaneustriatestacea/FuLi,HuChunxiang(NortheastForestryUniversity,Harbin150040,P.R.China)//JournalofNortheastF
4、orestryUniversity.一2014.42(7).一126—128.132Weadoptedleaf-dipmethodtoevaluateomethoateandcarbarylagainstthethirdinstarofMalacosomaneustriatestaceaandusedsepectrophotometrytoassesstheeffectofsublethalconcentrationofthetwopesticidesonactivitiesofcarboxyles.
5、teraseandglutathioneS.transferase.For24hand48htreatment,theLC,nofomethoatewere33.975and8.298mK·L~.a(chǎn)ndtheLC,nofcarbarylwere23.314and1.863mg·L~,respectively.TheactivitiesofCarEinlavaewereinhibitedatdifferentlevelwithin6-48htreatmentofsublethalconcentratio
6、nofomethoateandcarbary.ComparedtoCK。theactivitiesofCarEweredecreasedby51%and36%,respectively.a(chǎn)fter12htreatment,whichweredecreasedtothelowestleve1.Bythetreatmentofthesublethalconcentrationofomethoat.theactivityofGSTswasinhibitedfirstly.thenincreasedandin
7、.hibitedfinally.After24htreatmentwithsublethalconcentrationofomethoate.theactivitywasincreasedby1.37timescomparedtothatofCK,whichwasathighestleve1.Within6—12hoftreatmentwithsublethalconcentrationofcarbarv1.theactivityofGSTsinlarvaewasincreasedfirstly,an
8、dthendecreasedgradually.KeywordsMalacosomaneustriatestacea;Sublethaldoses:Carboxylesterase:GlutathioneS—transferase黃褐天幕毛蟲(chóng)(Malacosomaneustriatestacea)屬果、甲萘威的合理使用和黃褐天幕毛蟲(chóng)的綜合防治鱗翅目枯葉蛾科,其分布范圍廣,食性雜,危害重,主提供理論依據(jù)。要以幼蟲(chóng)危害柞樹(shù)、柳樹(shù)、楊樹(shù)、樺樹(shù)等闊葉樹(shù)_1],1