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1、·674·寧夏醫(yī)學(xué)雜志2014年8月第36卷第8期NingxiaMedJ，Aug．2014Vol36,No．Doi：10．13621／j．1001—5949．2014．08．0674·論著·入—鼠電點(diǎn)燃島葉致癇模型建立后神經(jīng)肽Ghrelin的表達(dá)劉諍，王峰，宋子木，劉陽，高攀，李揚(yáng)，孫濤[摘要]目的電點(diǎn)燃大鼠島葉建立致癇模型，檢測致癇大鼠神經(jīng)肽Ghrelin變化特征，并探討其意義。方法將雄性SD大鼠用數(shù)字表法隨機(jī)分為空白對照組、假手術(shù)組和點(diǎn)燃組，又將點(diǎn)燃組分為點(diǎn)燃后1、3、7d和10d4個(gè)亞組。立體定向下將電極植人大鼠島葉
2、，建立致癇模型，觀察大鼠行為學(xué)，監(jiān)測大鼠腦電情況；Nissl染色觀察海馬區(qū)神經(jīng)元變化情況，用放射免疫法(RIA)和免疫熒光法分別檢測血清和下丘腦中Ghrelin的表達(dá)水平。結(jié)果點(diǎn)燃后各亞組血清Ghrelin表達(dá)水平均降低，差異有統(tǒng)計(jì)學(xué)意義(P<0．05)；下丘腦Ghrelin表達(dá)也明顯減少，差異有統(tǒng)計(jì)學(xué)意義(P<0．05)；各時(shí)間亞組間比較差異無統(tǒng)計(jì)學(xué)意義(P>0．05)。結(jié)論電刺激島葉可建立穩(wěn)定的點(diǎn)燃模型，癲癇發(fā)作后Ghrelin水平明顯降低，血清Ghrelin水平可以反映下丘腦中相應(yīng)神經(jīng)肽的變化趨勢。[關(guān)鍵詞]Ghre
3、lin；癲癇；島葉；下丘腦；血清[中圖分類號]R473[文獻(xiàn)標(biāo)識碼]AExpressionofneuropeptidesghrelinininsularelectricalkindledratsmodelLIUZheng，WANGFeng，SONGZimu，Yang，GAOPan，LIYang，SUNTao．DepartmentofNeurosurgery，GeneralHospitalNingxiaMed&alUniversity，Yinchuan750004，ChinaCorrespondingauthor：SUNTa
4、o，Email：suntao@nxmu．edu．ell[Abstract]0bjectiveTosetuptheinsularelectricalkindledratsmodel，andtoobserveexpressionlevelofneuropeptidesghre—linandtoexploreitssignificance．MethodsThemaleSDratswererandomlydividedintotheblankgroup，thesham—operatedgroupandkindledgroup．Th
5、elatterweredividedto4subgroupsofld，3d，7dand10daftertheratshadbeenseizure．Bystereotacticoperation，electrodeswereplacedintheratsinsularandsetupthemode1．Changesofbehaviors，ECoGandnervecellthroughNisslstainingoftheratswereobservedsimultaneously．RIA(RadioimmunologyAssa
6、y)andImmunofluorescencewereusedtodetecttheexpressionsofGhre一1inintheserumandhypothalamusoftheepilepsyrats．ResultsFromthebehavior．theECoGandthepathologyinepilepticratscon—firmedthattheratsofelectricalkindledtoinsularcanbesuccessfullyestablishedexperimentalanimalmod
7、els．Comparedtotheblankgroup，thesham—operatedgroup，allkindledgroupsoftheseizureratshadadecreasedexpressionofghrelinthroughRIAdetectintheratsserumandImmunofluorescencedetectintheratshypothalamus．Therewasnostatisticaldifferenceamonganykindledgroups(P>0．05)．Conclusion
8、Theinsularhastheseizurepotency．AnaccuratedetectionofseHlmexpressionlevelofghrlinmayreflectthefluctu—ationtrendofneuropeptidesinhypothalamus．[Keywords]Gh
