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1、2O48實用醫(yī)學雜志2014年第30卷第13期右美托咪定對大鼠胚胎神經(jīng)干細胞增殖及分化的影響李紅英于衛(wèi)張建杏周國斌摘要目的：探討右美托咪定(dexmedetomidine，DEX)對大鼠胚胎神經(jīng)干細胞(NSCs)增殖及分化的影響。方法：從孕14—16dSD大鼠胚胎中分離NSCs并原代培養(yǎng)及Nestin鑒定。實驗分為對照組、低濃度及高濃度DEX組，培養(yǎng)液中分別加入終濃度為0、1、10ng／mLDEX。M1Tr法檢測NSCs活性；5一溴脫氧尿(Brdu)法觀察檢測NSCs增殖；Westernblot法檢測增殖期N
2、SCs中ERK1／2磷酸化：免疫熒光法標記神經(jīng)元細胞、星形膠質(zhì)細胞、少突膠質(zhì)細胞和神經(jīng)元干細胞觀察細胞分化。結(jié)果：分離培養(yǎng)的神經(jīng)干細胞95％以上呈Nestin陽性；低濃度DEX組M1Tr吸光值、Brdu陽性細胞及EKR1／2磷酸化水平較對照組明顯增加(P<0．O5)。而高濃度DEX組較對照組及低濃度DEX組明顯降低(P<0．05)；高、低濃度DEX組中NeuN、GFAP及MBP陽性細胞數(shù)較對照組明顯增加(P<0．05)，Nestin陽性細胞則顯著降低(P<0．05)。而高、低濃度DEX組間無明顯差異(P>0．
3、05)。結(jié)論：低濃度DEX誘導(dǎo)體外培養(yǎng)大鼠神經(jīng)干細胞增殖而高濃度DEX則抑制其增殖，其機制可能通過調(diào)節(jié)EKR1／2磷酸化；高、低濃度DEX均誘導(dǎo)神經(jīng)干細胞非選擇性分化關(guān)鍵詞右美托咪定；神經(jīng)干細胞：增殖：分化Efectofdexmedet0HIidineonproliferationanddiferentiationofratembryonicneuralstemcellsinvitroLIHong-ying，YUWei，ZHANG帆一xing，ZHOUGuo—bin．．GuangdongGeneralHosp
4、ital，GuangdongAcademyofMedicalSciences，Guangzhou510080，ChinaCorrespondingauthor：YUWeiE—moil：yw72tim@163．tom【Abstract】0bjectiveToinvestigatetheeffectofdexmedetomidine(DEX)onproliferationanddifferentiationofratembryonicneuralstemcellsinvitro．MethodsEmbryonicn
5、euralstemcellsoffetalSDratswereseparatedfromratswithgestationalageof14-16days，andunderwentprimaryculturefornestinexpression．Cellsweredividedintocontrolgroup，low-doseDEXgroup，andhigh—doseDEXgroup，whichwereculturedwith0，1and10ng／mLDEXrespectively．Cellviabilit
6、ywasdetectedbyMTFassay；theproliferationratewasestimatedbyBrdUincorporation；thephosphorylationofERK1／2intheproliferationphaseswasdetectedbywesternblot；thedifferentiationofneuronalcells，astrocytes，oligodendrocyteandneuralstemcellswereassessedbyimmunocytochemi
7、stryofcellspecificmarkers．ResultsMorethan95％oftheembryonicneuralstemcellsinprimaryculturewereNestin—positive．Cellviability，BrdU—positivecellsandphosphorylationofERK1／2inlow-doseDEXgroupincreasedsignificantly(P<0．05)，anddecreasedsignificantlyinhigh—doseDEXgr
8、oupcomparedwithcontrolgroupandlow—doseDEXgroup(P<0．05)．NeuN一，GFAP—andMBP—positiveceilsincreased(P<0．05)，whileNestin-positivecelldecreased(P<0．05)inbothlowandhighdoseDEXgroupcomparedwithcontrolgroup．How
