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1、·246·中國肺癌雜志2008年4月第11卷第2期ChinJLungCancer,April2008,Vol.11,No.2·臨床研究·趨化因子受體CCR7在NSCLC與轉(zhuǎn)移淋巴結(jié)中的表達(dá)關(guān)系及意義曾濤溫劍虎李醒【摘要】背景與目的惡性腫瘤的靶向轉(zhuǎn)移是近年來研究的熱點(diǎn)，研究已經(jīng)證實(shí)惡性腫瘤的轉(zhuǎn)移是一種非隨機(jī)的、有組織器官選擇性的高度組織化的過程，本研究通過對(duì)趨化因子受體CCR7在非小細(xì)胞肺癌及其轉(zhuǎn)移淋巴結(jié)中的表達(dá)狀況進(jìn)行分析，初步探討趨化因子受體CCR7在非小細(xì)胞肺癌中的表達(dá)及其與淋巴結(jié)轉(zhuǎn)移之間的關(guān)系和意義。方法用兔抗人CCR7抗體免疫組化染色檢測(cè)肺腺癌17例，鱗癌17例，腺鱗癌12例，大細(xì)胞肺
2、癌4例和轉(zhuǎn)移淋巴結(jié)28例中CCR7的表達(dá)情況。陰性對(duì)照標(biāo)本采用炎性假瘤5例，正常肺組織20例。結(jié)果判定在雙盲法下進(jìn)行，每張切片由兩名病理醫(yī)師分別判斷。結(jié)果1.CCR7在癌組織和轉(zhuǎn)移淋巴結(jié)中的表達(dá)高于正常肺組織（P<0.005）；2.轉(zhuǎn)移淋巴結(jié)中CCR7表達(dá)與癌組織中CCR7的表達(dá)相比無差異(P=0.177)；3.CCR7在癌組織中的表達(dá)與淋巴結(jié)轉(zhuǎn)移相關(guān),淋巴結(jié)轉(zhuǎn)移組高于無淋巴結(jié)轉(zhuǎn)移組(P=0.016)；4.隨著臨床分期的增加，CCR7表達(dá)有增高趨勢(shì)(P=0.003)。結(jié)論CCR7蛋白主要在肺癌腫瘤細(xì)胞與轉(zhuǎn)移淋巴結(jié)中表達(dá)，而在正常肺組織中未見表達(dá)，其表達(dá)水平可能與非小細(xì)胞肺癌淋巴轉(zhuǎn)移有關(guān)?！娟P(guān)鍵
3、詞】CCR7肺腫瘤腫瘤轉(zhuǎn)移【中圖分類號(hào)】R730.5DOI:10.3779/j.issn.1009-3419.2008.02.021ThevalueandassociationofCCR7expressioninNSCLCwithlymphnodemetastasis*ZENGTao,WENjianhu,LIXing*DepartmentofCardiothoracicSurgery，Mianyang404Hospital,Mianyang,Sichuan621000,ChinaCorrespondingauthor:ZENGTao:nhzengtao@yahoo.com.cn【Abstrac
4、t】BackgroundandObjectiveIthasbecameahotspotresearchaboutthetargetmetastasisofmalignanttumorinrecentyears.Ithasbeenproventhatmetastasisofmalignanttumorisanonrandombuthighly-organizedandselectiveprocess.TheaimofthisstudyisbyanalysingtheexpressionofCCChemokineReceptor7(CCR7)inpulmonarytumortissueandmet
5、astasizedlymphnodesinNSCLC,toexploretherelationshipbetweentheexpressionofCCR7inpulmonarytumortissueandmetastasizedlymphnodes,andexplorethesignificance.MethodsSABCimmunohitochemcalstainingwasusedtoinvestigatetheexpressionofCCR7byrabbitanti-humanCCR7monoclonalantibody,andthespecimenswere17casesofadeno
6、carcinoma,17casesofSquamouscellCarcinoma,12casesofAdenosquamousCarcinoma,4casesoflargecellcarcinomaand28casesofmetastasizedlymphnodesoflungcancer.Negativecontrolsectionsuse5casesofinflammatorypseudotumorand20casesofnormallungtissue.Twoindependentpathologistsobservedallthespecimensinthehighpowerfield
7、(×400)ofmicroscopebydoubleblindmethod.Results1.TheexpressionofCCR7inpulmonarytumortissuewasremarkablyhigherthannormallungtissue(P<0.005);2.TheexpressionofCCR7betweenpulmonarytumortissueandmetastasized
