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1、中華麻醉學(xué)雜志2014年7月第34卷第7期ChinJAnesthesiol，July2014，Vo1．34，No．7873·重癥醫(yī)學(xué)P物質(zhì)對新生大鼠心肌細(xì)胞缺氧復(fù)氧損傷的影響趙鑫陳璐郭政【摘要】目的探討P物質(zhì)對新生大鼠心肌細(xì)胞缺氧復(fù)氧損傷的影響。方法新生sD大鼠，1～3日齡，處死后取心室肌組織，分離心肌細(xì)胞，接種于6孔培養(yǎng)板中，培養(yǎng)72h。采用隨機(jī)數(shù)字表法，將心肌細(xì)胞分為4組(n=3)：對照組(C組)常規(guī)培養(yǎng)6h；缺氧復(fù)氧組(A／R組)進(jìn)行缺氧3h，復(fù)氧2h；P物質(zhì)組(sP組)給予終濃度為10一mol／L的P物質(zhì)孵育lh，行缺氧3h，復(fù)氧2h；P物質(zhì)+D．
2、sP組(sP+D．SP組)給予終濃度為10。mol／L的P物質(zhì)和終濃度為10mol／L的速激肽1型受體拮抗劑D．SP孵育1h，行缺氧3h，復(fù)氧2h。于復(fù)氧結(jié)束時(shí)，測定心肌細(xì)胞凋亡率和乳酸脫氫酶(LDH)活性。結(jié)果與c組比較，A／R組、sP組和sP+D—sP組細(xì)胞凋率和LDH活性升高(P<0．01)；與A／R組比較，sP組和sP+D．sP組細(xì)胞凋亡率和LDH活性降低(P<0．01)；與sP組比較，sP+D—SP組細(xì)胞凋亡率和LDH活性升高(P<0．01)。結(jié)論P(yáng)物質(zhì)可減輕新生大鼠心肌細(xì)胞缺氧復(fù)氧損傷，其機(jī)制與激活速激肽1型受體有關(guān)。【關(guān)鍵詞】P物質(zhì)；肌細(xì)胞，
3、心臟；細(xì)胞低氧；氧；嬰兒，新生EfectsofsubstancePonanoxia／reoxygenationinjurytocardiomyocytesofneonatalratsZhaoXin，ChenLu，GuoZheng．DepartmentofAnesthesiology，ShanxiMedicalUniversity，Taiyuan030001，ChinaCorrespondin~author：GuoZheng，DepartmentofAnesthesiology，ShanxiMedicalUniversitySecondHosptialofS
4、hanxiMedicalUniversity，Taiyuan030001，China，Email：guozheng713@163．corn【Abstract】ObjectiveToinvestigatetheeffectsofsubstancePonan0xia／re0xygenati0n(A／R)injurytocardiomyocytesofneonatalrats．MethodsCardiomyocytesofneonatalratswereisolatedfromSprague—Dawleyrats，aged1-3days，andwerecultu
5、redin6-wellplatesfor72h．Thecardiomyocyteswerethenassignedinto4groups(n=3each)usingarandomnumbertable：controlgroup(groupC)，A／Rgroup，substancePgroup(groupSP)andsubstanceP+D—SPgroup(groupSP+D—SP)．Thecellswereculturedroutinelyfor6hingroupCandthecellswereexposedto99．9％N2inanincubatorat
6、37ccfor3hfollowedbyreoxygenationfor2hintheothergroups．ThecellswereincubatedwithsubstancePwiththefinalconcentrationof10～mol／Lfor1hbeforean0xiaingroupSP．Thecellswereincubatedfor1hwithsubstancePwiththefinalconcentrationof10一mol／LandD。SP(aspecificantagonistofneurokinin．1receptor)witht
7、hefinalconcentrationof10“mol／LbeforeanoxiaingroupSP+D—SP．Theapoptosisrateandlactatedehydrogenase(LDH)activityweredetectedattheendofreoxygenationusingTUNELassayandLDHassaykit，respectively．ResultsComparedwithCgroup，theapoptosisrateandLDHactivityweresignificantlyincreasedinA／R．SPandS
8、P+D—SPgroups．ComparedwithA／Rgroup
