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1、CHINESEJ0URNALOFANAT0MYVo1．37No．22014解剖學雜志2014年第37卷第2期山楂葉總黃酮對血管性癡呆大鼠腦內(nèi)膽堿能活性及學習記憶的影響苗光新毛曉霞吳曉光陳志宏△曹慧王嬌檀榮方王妍(承德醫(yī)學院河北省中藥研究與開發(fā)重點實驗室，承德067000)摘要目的：探討山楂葉總黃酮(TFHL)對血管性癡呆大鼠空問學習記憶障礙的改善作用及對腦內(nèi)膽堿能系統(tǒng)功能活性的影響。方法：健康雄性SD大鼠8O只，隨機分為Sham、模型組、TFHL70mg／kg組、TFHL140mg／kg組和鹽酸多奈哌齊0．52mg／k
2、g組，每組16只。采用永久性雙側(cè)頸總動脈結(jié)扎法制備血管性癡呆大鼠模型，TFHL70、140mg／kg及鹽酸多奈哌齊0．52mg／kg組連續(xù)給藥36d。采用Morris水迷宮評價大鼠的空問學習記憶能力，H—E染色觀察大鼠海馬CAJ區(qū)神經(jīng)元形態(tài)及密度，酶聯(lián)免疫法檢測大鼠皮質(zhì)、基底節(jié)、海馬乙酰膽堿酯酶(AchE)和膽堿乙?；D(zhuǎn)移酶(ChAT)活性變化。結(jié)果：與Sham比較，血管性癡呆模型組大鼠學習記憶能力顯著降低，大鼠逃避潛伏期明顯延長，穿越平臺區(qū)次數(shù)顯著減少，平臺象限游泳距離百分比顯著降低；ChAT活性明顯降低，AChE活
3、性顯著增強。TFHL組縮短大鼠逃避潛伏期，增加大鼠穿越平臺次數(shù)及平臺象限游泳距離百分比，增強chAT活性，降低AChE活性。結(jié)論：TFHL可改善血管性癡呆大鼠膽堿能神經(jīng)系統(tǒng)的功能，從而提高血管性癡呆大鼠的學習記憶功能。關(guān)鍵詞山楂葉總黃酮；血管性癡呆；膽堿乙?；D(zhuǎn)移酶；乙酰膽堿酯酶ImpactoftotalflavoneofhawthornleafonlearningandmemoryabilityandintracephaliccholinergicactivityinratswithvasculardementiaM
4、iaoGuangmn，MaoXiaoxia，WuXiaoguang，ChenZhihonga，CaoHui，WangJiao，TanRongfang，WangYan(HebeiProvinceKeyLaboratoryofResearchandDevelopmentforTraditonalChineseMedicine，ChengdeMedicalCollege，Chengde067000，China)AbstractObjective：Toinvestigatetheeffectoftotalflavoneofha
5、wthornleaf(TFHL)onthetherapeuticeffectandmechanismofspatiallearningandmemoryimpairmentofthevasculardementiarat．Methods：80SDmaleratswererandomlydividedintoashamcontrolgroup，amodelgroup，TFHL70，140mg／kggroupanddonepezil0．52mg／kggroup(n一16)．Permanentbilateralcarotid
6、arteryligationwaspreparedtoestablishavasculardementiamode1．TFHL70，140mg／kggroupanddonepezil0．52mg／kggroupwereadministeredforconsecutive36days．Morriswatermazewasusedtoevaluatelearningandmemoryabilitiesofrats，andcolorimetricmethodwasemployedtodetectcortex，basalgan
7、gliaandhippocampusAChEandChATcontent．Results：Comparedwiththeshamcontrolgroup，learningandmemorycapacityofthevasculardementiamodelgroupsignificantlyreduced，andratescapeincubationperiodprolonged．Thenumberofcrossingtheplatformareawassignificantlyreducedandthepercent
8、ageoftargetquadrantdistancewaslowered；ChATactivitywassignificantlylowered，andAChEactivitywassignificantlyincreased．TFHLratswithvasculardementiaimprovedlearningandmemo
