PIWILl蛋白與DICER酶在肝細(xì)胞癌中的表達(dá)及意義-論文.pdf

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1、·9O8·中華肝膽外科雜志2013年12月第19卷第12期ChinJHepatobiliarySurg，December2013，Vo1．19，No．12·論著·PIWIL1蛋白與DICER酶在肝細(xì)胞癌中的表達(dá)及意義范正軍孫濤楊飛龍程波彭飛周闖【摘要】目的探討P1wII1蛋白與DICER酶在肝細(xì)胞癌組織中的表達(dá)及臨床意義。方法免疫組化檢測(cè)47例肝細(xì)胞癌標(biāo)本中PIWIL1與DICER的表達(dá)。蛋白印跡法檢測(cè)31例肝細(xì)胞癌組織中PIWIL1與DICER的表達(dá)。分析PIWII1與DICER表達(dá)及與臨床特征的關(guān)系。結(jié)果PIWlI1在癌組織中高表達(dá)，在正常肝組織中低表達(dá)

2、(P

3、carcinomaFANZheng—un，SUNTa0，yANGFei—long，CHENGBo，PENGFei，ZH0UChuang．eFirstAffiliatedHospital0fZhengzhouUniversity，Zhengzhou450052，ChinaC0rre聲。ngauthor：FANZheng—Un，Email：droider@163．com[Abstract]ObjectiveToexploretheexpressionofPIWIL1proteinandDICERenzymeinhepatocellularcarcinoma(HC

4、C)andtheirsignificance．MethodsImmunohistochemica1methodwasusedtodetecttheexpressionofPIWIL1andDICERin47casesofHCCandtheadjacentHCCtissues．WesternblotmethodwasusedtOdetecttheexpressionofPIWIL1andDICERin31casesoffreshHCCtissuesandtheiradjacentHCCtissues．TherelationshipbetweenPIWIL1an

5、dDICERandtheirrelationshipswereanalysedwithclinica1features．12casesofnorma1livertissueswereusedascontrolgroup．ResultsTheexpressionofPIWIL1washighinHCCbutlOWinnormal1ivertissues(P

6、velycorrelatedwithinvasiontoadjacenttissuesandhistologicaldiffer—entiation(Pd0．O5)．TheexpressionofDICERwasnegativelycorrelatedwithinvasiontotheadacenttissuesandhistologica1differentiation(Pd0．05)．ThereW38anegativecorrelationbetweenPIWIIlandDICER(P<0．05)．ConclusionsHighexpressionofP

7、IWII1andlow／missingexpressionofDICERwasrelatedtopathologicaldifferentiationandinvasionofadjacenttissues．[Keywords]Hepatocel1ularcarcinoma；Piwilikeprotein；Ribonuclease1I，DICER肝細(xì)胞癌(hepatoce11ularcarcinoma，HCC)具原(Piwi-likeprotein1，PIWILl／H1wI)為新發(fā)現(xiàn)有發(fā)病率高、難以早期發(fā)現(xiàn)、病死率高等特點(diǎn)。我國的惡性腫瘤基因蛋白。PIWII

8、1與多種腫瘤的發(fā)生每年因肝細(xì)胞癌死亡的人數(shù)在惡性腫瘤