喉鱗狀細(xì)胞癌中肝細(xì)胞生長(zhǎng)因子受體的表達(dá)及意義-論文.pdf

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1、中國(guó)煤炭工業(yè)醫(yī)學(xué)雜志2013年11月第16卷第11期·1775·喉鱗狀細(xì)胞癌中肝細(xì)胞生長(zhǎng)因子受體的表達(dá)及意義戴文成蔣斌韓靚摘要目的研究肝細(xì)胞生長(zhǎng)因子受體(c—Met)在喉鱗狀細(xì)胞癌患者組織及血漿中的表達(dá)，探討c—Met在喉鱗狀細(xì)胞癌發(fā)生、發(fā)展所起的作用。方法喉鱗狀細(xì)胞癌及喉乳頭狀瘤患者進(jìn)行調(diào)查，并且設(shè)立正常對(duì)照組，應(yīng)用酶聯(lián)免疫吸附法檢測(cè)各組血漿c—Met水平；應(yīng)用半定量RT—PCR法和免疫組織化學(xué)法分別檢測(cè)各組組織中c—MetmRNA及C—Met蛋白的表達(dá)。結(jié)果喉鱗狀細(xì)胞癌患者血漿中的e—Met水平明顯高于喉乳頭狀瘤患者和正常人群(均P<0．05)。喉鱗狀細(xì)胞癌組織中的C

2、—Met水平明顯高于喉乳頭狀瘤組織和正常組織(均P<0．05)。結(jié)論C—Met在喉鱗狀細(xì)胞癌組織及血漿中呈高表達(dá)，可能與喉鱗狀細(xì)胞癌的發(fā)生、發(fā)展有關(guān)。關(guān)鍵詞肝細(xì)胞生長(zhǎng)因子受體；喉鱗狀細(xì)胞癌；酶聯(lián)免疫吸附法；逆轉(zhuǎn)錄多聚酶鏈?zhǔn)椒磻?yīng)；免疫組織化學(xué)中圖分類號(hào)R739．65’文獻(xiàn)標(biāo)識(shí)碼B文章編號(hào)1007—9564(2013)11—1775—04D0I編碼10．11723／mtgyyx1007—9564201311009TIIEsTUDYFoRTHEEXPRESS10N0FHEPAToCYTEGR0WTHFACT0RRECEPToRITHESERUMANDTISSUESINPATIE

3、NTSWITHLARYNGEALSQUAM0USCELLCARCIN0MASDaiWencheng。JiangBin-HanLiang．DepartmentofHeadandNeckSurgery，NantongTumorHospital，Nantong226361，ChinaAbstractObjectiveTostudytheexpressionofhepatocytegrowthfactorreceptor(c—Met)intheserumandtissuesinpatientswithlaryngealsquamouscellcarcinomas。exploret

4、heroleofC—Metinthetumori—genesisandprogressionofhumanlaryngealsquamouscellcarcinomas．MethodsSixtypatientswithlaryn—gealsquamouscellcarcinomasorfortycasesoflaryngealpapillomawereselectedinauthorshospital-andfortyhealthpersonswereenrolledincontro1group．Theplasmac—Metconcentrationsinthemwasd

5、etec—tedbyenzyme—linkedimmunosorbentassay(ELISA)；theexpressionofe—MetmRNAandproteinweredetectedintissueofthembyusingsemi—quantitativereversetranscription—polymerasechainreactionfRT—PCR)andimmunohistochemistryrespectively．ResultsTheplasmac—Metconcentrationsinpatientswithlaryngealsquamousce

6、llcarcinomaswashigherthanthoseinlaryngealpapillomaandnormalcontrol、group(P

7、gealsquamouscellcarcinomasishigh，c—Metmaybeinvolvedinthetumorigenesisandprogressionoflaryngealsquamouscellcarcinomas．KeywordsHepatocytegrowthfactorreceptor；Laryngealsquamouscellcarcinomas；Enzyme—linkedim—munosorbentassay(ELISA)；Reversetranscription—polymerasechainre