不同負(fù)荷的游泳運(yùn)動(dòng)對(duì)大鼠認(rèn)知能力及海馬神經(jīng)疲勞的影響-論文.pdf

《不同負(fù)荷的游泳運(yùn)動(dòng)對(duì)大鼠認(rèn)知能力及海馬神經(jīng)疲勞的影響-論文.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。

1、中國(guó)實(shí)驗(yàn)診斷學(xué)2014年3月第18卷第3期一377一文章編號(hào)：1007—4287(2014)03—0377—03不同負(fù)荷的游泳運(yùn)動(dòng)對(duì)大鼠認(rèn)知能力及海馬神經(jīng)疲勞的影響劉忠民，高拮，牛娜，董煒達(dá)(吉林大學(xué)體育學(xué)院，吉林長(zhǎng)春130012)摘要：目的探討不同負(fù)荷游泳運(yùn)動(dòng)對(duì)大鼠學(xué)習(xí)記憶的認(rèn)知能力及海馬神經(jīng)型一氧化氮合酶(n-NOS)表達(dá)與疲勞機(jī)制。方法將4O只體重為350—400g的Wistar雄性大鼠分為對(duì)照組和每天進(jìn)行15mins、30mins、60rains的小中大負(fù)荷游泳運(yùn)動(dòng)的實(shí)驗(yàn)組；實(shí)驗(yàn)持續(xù)8周后采

2、用“Y迷宮”進(jìn)行學(xué)習(xí)記憶能力測(cè)試；利用S-P免疫組化染色法檢測(cè)海馬神經(jīng)n-NOS的表達(dá)水平。結(jié)果學(xué)習(xí)記憶的達(dá)標(biāo)次數(shù)和錯(cuò)誤次數(shù)，中等負(fù)荷組顯著低于對(duì)照組和大負(fù)荷組(P<0．05和P％0．01)；與對(duì)照組相比中、大負(fù)荷組海馬神經(jīng)n—NOS陽性表達(dá)均顯著提高(P<0．01)；與小負(fù)荷組比較，中負(fù)荷組和大負(fù)荷組均有顯著性差異(P

3、高；大運(yùn)動(dòng)負(fù)荷組學(xué)習(xí)記憶能力下降而n—NOS表達(dá)水平提高，其相互作用機(jī)制有待于深入研究。關(guān)鍵詞：游泳運(yùn)動(dòng)；學(xué)習(xí)記憶；海馬神經(jīng)；一氧化氮合酶中圖分類號(hào)：R338．3文獻(xiàn)標(biāo)識(shí)碼：ATheInfluenceofVariousSwimmingLoadsonRats’CognitiveCapabilityandHippocampalNeuronFatigueLIUZhong—min，GAOZhe，NIUNa，eta1．(PhysicalFpducationCollege，JilinUniversity，Cha

4、ngchun130012，China)Abstract：ObjectiveThepurposeistoexplorethefatiguemechanismofrats’cognitiveabilitytolearnandmemo—rizeandtheexpressionlevelsofhippocampalneuronn—NOSundervariousswimmingloads．Methods40Wistarmaleratsweighing350—-400garedividedintoacontro

5、lgroupand3experimentalgroupsthatswimundervariousloadsevery—-day，for15rain，30min，and60minrespectively．After8weeks，theratsreceiveabilitytestsoflearningandmemorizingthoughaY—mazeandthelevelsofhippocampalneuronn-NOSexpressionaremeasured，bymeansofummunitygr

6、oupdyeing．Resultsinthetimesofpassandfailure，thegroupundermoderateloadissignificantlylowerthanthecontrolgroupandthegroupunderlargeload(P<0．05，P<0．01)；comparedwiththecontrolgroup，positiveexpressionofhippocampalneuronn-NOSlevelsofthegroupsundermoderateand

7、largeloadsincreasesignificantly(P％0．01)；thereisasignificantdifferencebetweenthegroupundersmallloadandgroupsundermoderateandlargeloads(P％0．05，P<0．01)；thereisalsoasignificantdifferencebetweenthegroupundermoderateloadandtheoileunderlargeload(PdO．O1)．Concl

8、usionswimmingofmoderateloadcaneffectivelyimproverats’abilitytolearnandmemorize；thelevelsofhippocampalneuronn—NOSexpressionincreasesignificantlyalongwiththeincreaseoftheloads：theabilitytolearnandmemorizeofthegroupunderlargeloaddecreasesw