流式細(xì)胞術(shù)分析牛乳鐵蛋白素對Jurkat細(xì)胞增殖、分化的影響.pdf

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1、v，nv．chinadairy．net中國乳品工業(yè)dozgrpgy@163．com,jryINDUSTRY流式細(xì)胞術(shù)分析牛乳鐵蛋白素跏urkat細(xì)胞增殖、分化的影響王靜，趙寧，李萌，張艷杰。，張書義，滕國新，宋真，劉寧Iab,2許曉曦(1．東北農(nóng)業(yè)大學(xué)a．食品學(xué)院，b．乳品科學(xué)教育部重點(diǎn)實(shí)驗(yàn)室，哈爾濱150030；2．黑龍江省乳品工業(yè)技術(shù)開發(fā)中心，哈爾濱150028；3．上海晨冠乳業(yè)有限公司，上海201401；4．全國畜牧總站，北京100125；5．北京城市學(xué)院，北京100094)摘要：采用流式細(xì)胞術(shù)分析了牛乳鐵蛋白素(BovineLactoferrcin，LfcinB)5

2、@Jurkat胞增殖周期、細(xì)胞表面分化抗原表達(dá)水平的影響。結(jié)果表明：LfcinB處~Jurkat胞后，隨著Lfcin13濃度增加，處于G／G。期的lurkat胞百分率明顯上升，s期的細(xì)胞百分率明顯下降，細(xì)胞表面分化抗原CD1lb表達(dá)水平明顯升高；此外，利用CCK一8法、NBTi~原反應(yīng)觀察LfcinB5@Jurkat胞增殖、分化的影響，發(fā)現(xiàn)細(xì)胞增殖抑制率顯著上升．NBT還原反應(yīng)OD值明顯提高，具有劑量和時(shí)間依賴性。以上結(jié)果說明，LfcinB~g夠通過細(xì)胞周期阻滯抑制lurkat~胞增殖．并能誘導(dǎo)其分化關(guān)鍵詞：牛乳鐵蛋白素；lurkat~胞；增殖；細(xì)胞周期；分化；分化抗原中圖

3、分類號(hào)：Q93—33文獻(xiàn)標(biāo)識(shí)碼：A文章編號(hào)：1001—2230(2014)05—0009—04EfectsontheproliferationanddiferentiationofBovineLactoferrcintoJurkatcellbyflowcytometricanalysesWANGJing，zHAoNingbb，LIMeng2，zHANGYan-jie。，zHANGShu—yi，TENGGuo-xin．SONGZhen，LIUNinglab,2XUXiao-xi(1．NortheastAgriculturalUniversity，a．CoUegeofFoodS

4、cience，b．KeyLaboratoryofDairyScience，MinistryofEduca—tion，Harbin150030，China；2．HeilonNiangDairyIndustryTechnicalDevelopmentCenter，Harbin150028，China；3．ShanghaiChenguanDairyCo．LTD．，Shanghai201401，China；4．NationalAnimalHusbandryService，Beijing100125．China；5．BeijingCityCollege，Beijing100094，C

5、hina)Abstract：Intheexperiments，flowcytometricanalyseswereusedtoresearchtheefectsofLfcinBonJurkatcellcycleandcelsurfacedifer-entiationantigenexpressionleve1．TheresultsshowedthatafterLfcinBtreatingJurkatcell，asLfCinBconcentrationincreasing，thecelpercent—ageinG1／Gophasewasincreasedsignificant

6、ly，andthecellpercentageinSphasewasdecreasedsignificantly，aswelaScellsurfacediferentia—tionantigenCD1lbexpression1eve1improvedobviously；inaddition．theefectsofLfcinBoncelproliferationanddifierentiationwereob—servedbvCCK一8methodandNBTreductionreaction，andfoundthatcelproliferationinhibitionrat

7、eincreasedsignificantly，andtheODvalueofNBTreductionreactionimprovedobviously，inadose—dependentandtime—dependentmanner．TheresultsaboveindicatedthatLfcinBcouldblockceHcycleinG1／G0phasetoinhibitJurkatcellproliferationandcouldinduceJurkatceldiferentiation．Keywords