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1、第42卷第5期東北林業(yè)大學學報V01.42No.52014年5月JOURNALOFNORTHEASTFORESTRYUNIVERSITYMay2014重瓣玫瑰誘導抗性對雙斑螢葉甲成蟲解毒酶的影響)張鑫乾嚴俊鑫楊杰瑩麻志爽舒文博孟慶德(東北林業(yè)大學,哈爾濱,150040)摘要為研究重瓣玫瑰(Rosarugosa‘plena’)的誘導抗蟲性,采用茉莉酸(JA)、茉莉酸甲酯(MeJA)、茉莉酮(Z-jasmone)、水楊酸(SA)誘導處理重瓣玫瑰植株,研究雙斑螢葉甲(Monoleptahieroglyphica(Motschulsky))成蟲取食不同化舍物誘導的重瓣玫瑰后,其堿性磷酸酯
2、酶(AKP)、酸性磷酸酯酶(ACP)、谷胱甘肽s一轉移酶(GSTs)、羧酸酯酶(CarE)、乙酰膽堿酯酶(AChE)的變化。結果表明:取食不同化合物誘導的重瓣玫瑰后,雙斑螢葉甲成蟲AKP活性先升高后降低,ACP活性均受到不同程度的抑制:取食MeJA處理植株的試蟲GSTs活性在l~5d顯著低于對照;取食JA、MeJA、Z—jasmone處理植株的試蟲CarE活性在第1天受到顯著抑制。MeJA在第l、3天誘導的植株抗性對試蟲CarE活性均有顯著抑制作用:JA誘導的植株抗性對試蟲AChE活性有短暫的抑制作用。JA、MeJA誘導的重瓣玫瑰對雙斑螢葉甲成蟲解毒酶有較強的抑制作用關鍵詞誘導抗
3、性;雙斑螢葉甲;解毒酶分類號s685.99:s763.3EfectsofInducedResistanceofRosarugosa‘plena’ontheActivitiesofDetoxifyingEnzymesinMonoleptahiero.glyphica(Motschulsky)/ZhangXinqian,YanJunxin,YangJieying,MaZhishuang,ShuWenbo,MengQingde(North·eastForestryUniversity,Harbin150040,P.R.China)//JournalofNortheastForestry
4、University.-2014,42(5).一125—128,148AftersprayingJA,MeJA,Z-jasmoneandSA,theactivitiesofalkalinephosphatase(AKP),acidphosphatase(ACP),glutathioneS.transferases(GSTs)。carboxylesterase(CarE),andacetvlch0linesteras(AChE)ofMonoleptahi—eroglyphica(Motschulsky)weremeasuredtostudytheinducedresistancet
5、oinsectsofRosarugosa‘plena’.Afterfeedingtheplantsinducedbydifferentcompounds.theAKPactivitiesofhieroglyphica(Motschulsky)increasesfirstandthendecreasesafterfeeding.ACPactivitiesaresignificantlyinhibited.GSTsactivitiesofhieroglyphica(Motschulsky)aresignificantlylowerthanthatofcontrolafterfeedi
6、ngplantstreatedwithMeJAinthefirstfivedays.CarEactivitiesaresig—nificantlyinhibitedafterfeedingplantstreatedwithJA,MeJA,Z-jasmoneinthefirstday,andCarEactivitiesaresignifi—cantlyinhibitedafterfeedingplantstreatedwithMeJAonthefirstandthirdday.AChEactivitiesofM.hieroglyphica(Motschulsky)areinhibi
7、tedafterfeedingplantstreatedwithJAforashorttime.TheplantstreatedwithJAandMeJAcaninhibitdetoxifyingenzymesactivitiesofM.hieroglyphica(Motschulsky).KeywordsInducedresistance:Monoleptahieroglyphica(Motschulsky);Detoxifyingenzymes植物的誘導抗性是指植物在遭到外界