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1、中國(guó)雇爹c互握2014,30(13):131—136ChineseAgriculturalScienceBulletin‘冬棗’子代種子萌發(fā)及下胚軸誘導(dǎo)愈傷組織研究金琪,王巖。,史良,王歡,張智勇,李穎岳‘(’北京林業(yè)大學(xué)生物科學(xué)與技術(shù)學(xué)院林木育種國(guó)家工程實(shí)驗(yàn)室/林木與花卉育種教育部重點(diǎn)實(shí)驗(yàn)室,北京100083,。北京市南口農(nóng)場(chǎng),北京102202)摘要:為實(shí)施‘冬棗’遺傳改良方案,以采自山西柳林自然授粉獲得的‘冬棗’子代種子為材料,測(cè)量種子橫、縱徑、種仁率等基本形態(tài)指標(biāo),并進(jìn)一步研究種子萌發(fā)最適條件和下胚軸誘導(dǎo)愈傷組織適宜培養(yǎng)基。結(jié)果表明:(1)‘冬棗’子代種子
2、橫徑為(8.62+0.05)mill,縱徑為(17.954-0.05)m/n,千粒重為(54.274-4.62)g,種仁率為(64.174-3.82)%,‘冬棗’屬于種仁率較高的棗樹品種。(2)種仁在合有GA30.4g/L的萌發(fā)培養(yǎng)基中預(yù)處理24h后接種到萌發(fā)培養(yǎng)基上,最有利于種仁萌發(fā),萌發(fā)率可達(dá)100%;液體萌發(fā)培養(yǎng)基稍好于固體萌發(fā)培養(yǎng)基,但未達(dá)顯著水平。(3)下胚軸誘導(dǎo)愈傷組織的適宜培養(yǎng)基為1/2MS+TDZ1.0mg/L+蔗糖30g/L,誘導(dǎo)率為(60.00-~3.02)o/0。關(guān)鍵詞:‘冬棗’;種子萌發(fā);下胚軸;愈傷組織中圖分類號(hào):$722文獻(xiàn)標(biāo)志碼:A
3、論文編號(hào):2013—2639SeedGerminationandHypocotylCallusInductionofZizipbusjujuba‘Dongzao’JinQi,WangYan,ShiLiang2,WangHuan,ZhangZhiyong,LiYingyue。(NationalEngineeringLaboratoryforTreeBreeding/KeyLaboratoryofGeneticsandBreedinginForestTreesandOrnamentalPlantsofMinutryofEducation,BeijingForestr
4、yUnivemity,Beijing100083;2Beij'ingNankouStateFarm,Beijing102202)Abstract:TheseedsofZizipbusjujuba‘Dongzao’fromShanxiwerecollectedtomeasurethemorphologicalindexes.Thetransversediameterandveaicaldiameterweremeasured,thethousandkernelweight(TKW)wasweighted,andtherateofkernelwascounted.T
5、hentheoptimalconditionforseedgerminationwasstudiedandtheappropriatemediumforhypocotylcallusinductionwasselected.Theresultswereasfollows:(1)Thetransverse,verticaldiameteroftheseedwere(8.62+0.05)mmand(17.95±0.05)mm,respectively;TKWwas(54.27±4.62)g;therateofkernelwas(64.17±3.82)%.So‘Don
6、gzao’wasoneofthehighratevarietiesofZizipbusjujube.(2)Theseedgerminationcouldreach100%onmediumwithGA30.4g/Lfor24hpretreatment.(3)Thehighesthypocotylcallusinductionrate[(60.00~3.02)%】wasobtainedon1/2MSbasalmediumsupplementedwith1.0mg/LTDZand30g/Lsucroseafter14daysincubationindarkness.K
7、eywords:Zizipbusjujuba‘Dongzao’;seedsgermination;hypocotyl;callus0引言維生素,還含有多種多糖、微量元素及抗癌物質(zhì)環(huán)磷酸棗(Ziziphusjujuba)為鼠李科(Rhamnaceae)棗屬腺苷等藥用成分,有很高的食療價(jià)值和保健功效,深受(Ziziphus)植物,是原產(chǎn)于中國(guó)的特色優(yōu)勢(shì)經(jīng)濟(jì)樹種,中國(guó)廣大消費(fèi)者的喜愛?!瑮棥?Ziziphusjujuba其味道鮮美,果形美觀,富含人體所需的多種氨基酸和‘Dongzao’)口感鮮嫩多汁,風(fēng)味酸甜適口,是鮮食品種基金項(xiàng)目:林業(yè)公益性行業(yè)專項(xiàng)項(xiàng)目“棗新品種
8、選育和產(chǎn)業(yè)升級(jí)關(guān)鍵技術(shù)研