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《膜突蛋白促進(jìn)乳腺癌細(xì)胞侵襲轉(zhuǎn)移的機(jī)制研究.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在應(yīng)用文檔-天天文庫(kù)。
1、復(fù)里虧搪醫(yī)學(xué)版FudanUnivMedSciZ,，U)13N。u．‘，’4葉。u(6u)679UnivJMed膜突蛋白促進(jìn)乳腺癌細(xì)胞侵襲轉(zhuǎn)移的機(jī)制研究倪翔邵志敏(復(fù)旦大學(xué)附屬腫瘤醫(yī)院乳腺外科上海200032)【摘要】目的探討膜突蛋白與乳腺癌細(xì)胞侵襲轉(zhuǎn)移的關(guān)系。方法應(yīng)用免疫組織化學(xué)法、RT—PCR和Westernblot等技術(shù)，檢測(cè)轉(zhuǎn)移與非轉(zhuǎn)移性乳腺癌組織以及不同轉(zhuǎn)移潛能人乳腺癌細(xì)胞系中膜突蛋白的定位及表達(dá)；應(yīng)用RNAi技術(shù)結(jié)合體外侵襲運(yùn)動(dòng)實(shí)驗(yàn)，探討膜突蛋白缺失對(duì)乳腺癌細(xì)胞侵襲運(yùn)動(dòng)能力的影響及相關(guān)分子機(jī)制。結(jié)果免疫組織化學(xué)結(jié)果顯示，膜突蛋白定位于細(xì)胞膜與細(xì)胞質(zhì)
2、，且在轉(zhuǎn)移組中的表達(dá)高于非轉(zhuǎn)移組，差異具有統(tǒng)計(jì)學(xué)意義(P=0．001，=17．536)。Westernblot結(jié)果顯示，膜突蛋白的表達(dá)與乳腺癌細(xì)胞轉(zhuǎn)移潛能呈正相關(guān)。此外，Transwell實(shí)驗(yàn)結(jié)果顯示，膜突蛋白表達(dá)缺失顯著抑制了乳腺癌細(xì)胞的侵襲和遷移能力，且對(duì)侵襲潛能高的乳腺癌細(xì)胞的抑制更為顯著。膜突蛋白缺失后，細(xì)胞中MTDHmRNA與nm-23mRNA水平明顯降低；vimentin蛋白表達(dá)增加、E—cadherin和MMP一9蛋白表達(dá)降低，差異具有統(tǒng)計(jì)學(xué)意義(P<0．05)。結(jié)論膜突蛋白高表達(dá)與乳腺癌轉(zhuǎn)移潛能增強(qiáng)呈正相關(guān)，可能通過(guò)影響MTDH和nm-23表
3、達(dá)、增強(qiáng)上皮間質(zhì)轉(zhuǎn)化、增加MMP一9分泌等來(lái)增強(qiáng)乳腺癌細(xì)胞的侵襲轉(zhuǎn)移能力?！娟P(guān)鍵詞】乳腺癌；腫瘤轉(zhuǎn)移；膜突蛋白；侵襲；轉(zhuǎn)移【中圖分類(lèi)號(hào)】R73—37【文獻(xiàn)標(biāo)志碼】Adoi：10．3969／i．issn．1672—8467．2013．06．009RelationshipbetweenmoesinexpressionandbreastcancermetastasisNIXiang，SHAOZhi—mink(DepartmentofBreastSurgery，ShanghaiCancerCenter，F(xiàn)udanUniversity，Shanghai200032，C
4、hina)[Abstract]ObjectiveToexploretheinternalrelationshipbetweenmoesinexpressionandbreastcancermetastasis．MethodsImmunohistochemistry(IHC)，RT_PCRandWesternblotwere"usedtoidentifymoesinexpressioninbreastcancertissuesamplesandaseriesofhumanbreastcancercel1lineswithdifferentmetastaticp
5、otentials．RNAitechniquecombinedwithinvasionandmotilityassayinvitrowerealsousedtorevealtheinterrelationshipbetweenmoesindeficiencyandinvasionandmotilityabilityofbreastcancercells．ResultsIHCanalysisshowedthatmoesinweremainlydistributed0nthecellmembraneandcytoplasm．Itsexpressionwashig
6、herinmetastaticbreastcancertissuesamplesthaninnon-metastaticones(P0．001，y。=17．536)．Westernblotresultsrevealedpositivecorrelationbetweenmoesinexpressionandmetastaticpotentialofbreastcancercells．TheresultsofTranswellexperimentdisplayedthatmoesinexpressionsilencingremarkablyinhibitedi
7、nvasionandmotilityabilityofbreastcancercells，especiallythecellswithhighmetastaticpotentia1．Aftermoesinexpression，thelevelsofMTDHmRNAandnm-23mRNAinbreastcancercells(P<0．05)significantlydecreased，whilevimentinexpressionincreased，andtheexpressionsofBcadherinandMMP-9decreased．Conclusio
8、nsMoesinover—expressionpre
