資源描述：
《purification and some properties of low-molecular-weight extreme halophilic》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在工程資料-天天文庫(kù)。
1、GModelMOLCAB-2345;No.ofPages7ARTICLEINPRESSJournalofMolecularCatalysisB:Enzymaticxxx(2011)xxx–xxxContentslistsavailableatSciVerseScienceDirectJournalofMolecularCatalysisB:Enzymaticjournalhomepage:www.elsevier.com/locate/molcatbPuri?cationandsomepropert
2、iesoflow-molecular-weightextremehalophilicxylanasefromChromohalobactersp.TPSV101B.aaba,?Prakash,M.Vidyasagar,S.K.Jayalakshmi,K.SreeramuluaDepartmentofBiochemistry,GulbargaUniversity,Gulbarga585106,Karnataka,IndiabAgriculturalResearchStation,Universityo
3、fAgriculturalSciences-Raichur,Gulbarga585106,IndiaarticleinfoabstractArticlehistory:Anextremehalophilicxylanasewaspuri?edfromculturesofChromohalobactersp.TPSV101byultra?l-Received18February2011tration,hydroxylapatiteandgel?ltrationchromatography.SDS-PA
4、GEofthexylanaseshowedanapparentReceivedinrevisedformhomogeneity?Septemberandmolecularweightof15kDa.ThexylanasehadmaximumactivityatpH9.0and65C201124inthepresenceof15–25%NaClandwasstableintherangeofpH7.0–9.0,temperaturebetween50andAccepted5October201170?
5、C.?AvailableonlinexxxTheenzymewasstableat50–65Cfor1hretaining100%activityandbyretaining60%activityat?2+2+2+80C.ThexylanasewascompletelyinhibitedbyHgionsandwaspartiallyinhibitedbyCa,Cuand2+2+2+2+Keywords:Pbions,whereasZn,MnandCoionsenhanceditsactivity.B
6、othEGTAandEDTAenhanceditssp.activity.Itwasactiveinsolutionscontainingwater-insolubleorganicsolventsandosmolytes.KineticTPSV101ChromohalobacterexperimentsindicatedthattheenzymehadKXylanasemandVmaxvaluesof0.2mg/mland1.17mol/ml/minforoatOsmolytesspeltxyl
7、an.Themajorproductsoftheoatspeltxylanhydrolysiswerexyloseandxylobiose;afterprolongedincubationxylosewasthemajorendproduct.OatspeltxylanXylobiose?2011ElsevierB.V.Allrightsreserved.1.IntroductionWhiletherehavebeennumerousreportsonextracellularxylanasesfr
8、omnon-halophilicbacteria,verylimitedinforma-Hemicellulosesarelow-molecular-weightpolysaccharidestionisavailableonxylanasesfromhalophilicspecies.Xylanolyticfoundincloseassociationwithcelluloseandligninintheplantactivitieshavebeenreported
