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《molecular characterization of mulberry》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在工程資料-天天文庫(kù)。
1、ResearchArticleReceived:5February2011Revised:16August2011Accepted:11October2011PublishedonlineinWileyOnlineLibrary:20December2011(wileyonlinelibrary.com)DOI10.1002/jsfa.4749Molecularcharacterizationofmulberry(Morusspp.)genotypesviaRAPDandISSRMuzaffer˙Ipek,a?Lut?Pirlak¨aandSalihKafkas
2、bAbstractBACKGROUND:Inrecentyears,DNA-basedmarkershavebeenusedquiteextensivelybecauseoftheirmanyadvantagesoverthetraditionalmorphologicalandbiochemicalmarkers.Manystudieshaveshownthatmolecularmarkersareusefulindelineatingthegeneticrelationshipsamongcloselyrelatedmulberrygenotypesandc
3、ultivars.Thus,inthepresentstudy,polymerchainreactionbasedDNA?ngerprintingtechniqueswereusedtoinvestigatethegeneticrelationshipsamongmulberrygenotypesgrowingindifferentagro-climaticregionsofTurkey.RESULTS:20RAPDprimersgeneratedatotalof173bands,ofwhich157(90.75%)werepolymorphic.Asfor11
4、ISSRprimers,124bands(96.55%)werepolymorphicinatotalof128.ThesimilarityindexforRAPDtechniquerangedbetween0.240.98;25˙Is203with25˙Is112werefoundtobetheclosestgenotypes,while24Ke10and25˙Is123werethemostdistantones.AccordingtotheISSRresult,thegeneticsimilarityindexchangedbetween0.21095;2
5、5˙Is203with25˙Is112genotypesweretheclosest,while25˙Is08and01KaD2werethemostdistantones.CONCLUSION:TheRAPDandISSRmarkerswerefoundtobepromisingforassessinggeneticdiversityinmulberrygenotypes.c2011SocietyofChemicalIndustryKeywords:RAPD;ISSR;mulberry;geneticsimilarityINTRODUCTIONcharact
6、ershavebeenpoorlyconducted.Ifhigh-yieldingandMulberriesbelongtothegenusMorusintheMoraceaefamily.qualityfruitphenotypeswereselectedandthenpropagated,About12mulberryspeciesarecultivatedworldwidefortheirmulberryproductioncouldbeincreased.foliage,fruit,timberorforornamentaluse.Amongthem,
7、MorusInrecentyears,DNA-basedmarkershavebeenusedquiteextensively5becauseoftheiradvantagesovertraditionalmorpho-alba,M.rubraandM.nigrahavebeenusedfortheirfruitsinbiochemicalmarkers.6,7MorphologicalmarkersarefewandmightsouthwestChinaintheFarEast(M.alba),inNorthAmerica(M.rubra)andinCentr
8、alAsia,Anato
