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1、中國水稻科學(ChinJRiceSci),2015,29(2):119-125http://www.ricesci.cnDOI:10.3969/ji.ssn.1001G7216.2015.02.002119水稻抽穗揚花期耐熱QTL(qHTH5)定位及其遺傳效應分析曹志斌謝紅衛(wèi)聶元元毛凌華李永輝蔡耀輝?(江西省超級水稻研究發(fā)展中心/國家水稻工程實驗室(南昌),南昌330200;?通訊聯(lián)系人,EGmail:cyhui123000@sina.com)MappingaQTL(qHTH5)forHeatToleranceattheHeadingStageonRiceChromosome5and
2、ItsGeneticEffectAnalysisCAOZhiGbin,XIEHongGwei,NIEYuanGyuan,MAOLingGhua,LIYongGhui,CAIYaoGhui?(LaboratoryofRiceNationalEngineering,JiangxiResearchandDevelopmentCenterofSuperRice,Nanchang330200,China;?Correspondingauthor,EGmail:cyhui123000@sinac.om)CAOZhibin,XIEHongwei,NIEYuanyuan,etal.MappingaQ
3、TL(qHTH5)forheattoleranceattheheadingstageonricechromosome5anditsgeneticeffectanalysis.ChinJRiceSci,2015,29(2):119G125.Abstract:UsinganinterspecificnearGisogeniclinederivedfromOryzasativassp.indica(Shuhui527)×O.rufipogonGriff.(YuanjiangcommonwildriceHehuatang3)asmaterials,aQTLforheattoleranceat
4、theheadingstageintheheatGtolerantnearGisogenicricelineYJ10G03G01wasanalyzed.Atotalof360simplesequencerepeat(SSR)markersevenlydistributedacross12chromosomeswereusedtodetectpolymorphismsbetweenYJ10G03G01andindicarestorerShuhui527,andninewerepolymorphic.Singlemarkeranalysisrevealedthatmarkersonchr
5、omosome5wereassociatedwithheattoleranceattheheadingstageusingseedsettingpercentageasindexforheattolerance.ByintervalmappingusingF2population(1,027plants)fromYJ10G03G01×Shuhui527,aQTLforheattolerancewasdetectedontheshortarmofchromosome5.TheQTLexplained8.6%and19.4%ofphenotypicvariancesintheF2andF
6、3generationsunderheatstress(artificialclimatechamber),respectively.Recombinantplantswerescreenedfortwoflankingmarkers,RM7320andRM7444,inF3generation,andsubstitutionmappingsuggestedthattheQTLwaslocatedinanintervalofabout304.2kbbetweenmarkersRM592andRM17921,wedesignatedtheQTLasqHTH5(quantitativet
7、raitlocusforheattoleranceattheheadingstageonchromosome5).Keywords:rice(OryzasativaL.);heattolerance;QTL;genemapping曹志斌,謝紅衛(wèi),聶元元,等.水稻抽穗揚花期耐熱QTL(qHTH5)定位及其遺傳效應分析.中國水稻科學,2015,29(2):119G125.摘要:對元江普通野生稻(OryzarufipogonGriff.)(簡稱元江普野)荷花塘3