陸地棉ssr標(biāo)記遺傳多樣性及其與農(nóng)藝性狀的關(guān)聯(lián)分析

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1、棉花學(xué)報(bào)CottonScience2014袁26淵5冤院420~430陸地棉SSR標(biāo)記遺傳多樣性及其與農(nóng)藝性狀的關(guān)聯(lián)分析*郭志軍袁趙云雷袁陳偉袁李運(yùn)海袁王紅梅袁龔海燕袁桑曉慧淵中國(guó)農(nóng)業(yè)科學(xué)院棉花研究所/棉花生物學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室袁河南安陽(yáng)455000冤摘要院分析陸地棉栽培種遺傳多樣性袁通過(guò)關(guān)聯(lián)分析尋找與棉花農(nóng)藝性狀相關(guān)聯(lián)的分子標(biāo)記袁為分子標(biāo)記輔助選擇育種和提高棉花育種效率奠定基礎(chǔ)遙本文采用74個(gè)Simplesequencerepeat(SSR)標(biāo)記對(duì)172份陸地棉栽培種的基因組變異進(jìn)行掃描袁使用NTSYS-pc2.20進(jìn)行聚類袁分析該群體遺傳多樣性

2、曰利用Structure2.3.4軟件分析群體結(jié)構(gòu)袁在此基礎(chǔ)上結(jié)合田間表型數(shù)據(jù)袁采用Tassel2.1的一般線性模型淵Generallinearmodel袁GLM冤進(jìn)行關(guān)聯(lián)分析袁定位與農(nóng)藝性狀相關(guān)的QTLs遙74個(gè)標(biāo)記共檢測(cè)到148個(gè)多態(tài)性位點(diǎn)袁涉及246個(gè)等位變異袁變異范圍2～7個(gè)袁平均等位變異數(shù)為3.32曰引物的多態(tài)性信息含量淵PIC冤為0.0281～0.3733袁平均值為0.2370曰遺傳相似系數(shù)變異在0.2816～1袁平均值為0.5369袁平均遺傳相似系數(shù)為0.5369袁表明我國(guó)陸地棉遺傳基礎(chǔ)狹窄袁盡管國(guó)外及西北內(nèi)陸棉區(qū)部分材料具有較豐富

3、的遺傳變異遙聚類分析將該群體劃分為12個(gè)亞群袁不同棉區(qū)的材料交叉分布袁且聚類結(jié)果基本與系譜吻合遙群體結(jié)構(gòu)分析卻將172份供試材料劃分為3個(gè)亞群曰通過(guò)關(guān)聯(lián)分析袁發(fā)現(xiàn)30個(gè)位點(diǎn)與鈴重堯衣分堯黃萎病抗性顯著相關(guān)淵＜0.05冤袁各位點(diǎn)對(duì)表型變異貢獻(xiàn)率為2.24%～5.27%遙關(guān)鍵詞院陸地棉曰關(guān)聯(lián)分析曰SSR曰遺傳多樣性曰群體結(jié)構(gòu)中圖分類號(hào)院S562.035文獻(xiàn)標(biāo)志碼院A文章編號(hào)院1002-7807淵2014冤05-0420-11*GuoZhijun,ZhaoYunlei,ChenWei,LiYunhai,WangHongmei,GongHaiyan,Sa

4、ngXiaohui(455000,)Toprovideageneticbasisformolecular-assistedbreedingandtoimprovetheefficiencyofbreeding,simplesequencerepeat(SSR)markersassociatedwithagronomictraitswerescreenedandthegeneticdiversityofcultivateduplandcottonwasanalyzed.In172uplandcottoncultivars,74SSRmarkersw

5、ereassayedforpolymorphism,andclusteranalysiswasperformedonthegeneticsimilaritymatrixusingNTSYS-pc2.20softwaretoascertainthediversity.Basedontheanalysisofpopulationstructure,anassociationanalysisbetweenSSRmarkersandthreeagronomictraitswereperformedusingtheTassel2.1GLM(generall

6、inearmodel)program.The74SSRmarkersincluded246allelesbelongto148loci袁withanaverageof3.32allelesperSSRmarker(range,2-7).Theaveragepolymorphicinformationcontent(PIC)forpermarkerwas0.2730(range,0.0281-0.3733),andthemeangeneticsimilaritywas0.5369(range,0.2816-1).Thisrevealedlowlev

7、elsofuplandcottongeneticdiversityinChina,althoughsomeoverseavarietiesandsomevarietiesofthenorthwestinlandcottonshowedabundantgeneticvariation.Clusteranalysisshowedthatalltheaccessionscouldbedividedinto12subgroups,whichwasinagreementwiththepedigreebutnotwiththeorigin.Structure

8、analysisindicatedthatthe172lineswereassignedtothreesubstructures.Oft