玉米rca基因克隆及表達分析

《玉米rca基因克隆及表達分析》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學術(shù)論文-天天文庫。

1、玉米RCA基因克隆及表達分析玉米RCA基因克隆及表達分析印志同印志同ztyin@yzu.edu.cn揚州大學農(nóng)學院玉米遺傳育種課題組揚州大學農(nóng)學院玉米遺傳育種課題組?改善作物光合性能是提高產(chǎn)量的重要途徑之一?Rubiscoactivase(RCA)catalyzestheactivationofRubiscoinvivoandplaysacrucialroleinregulatingplantgrowth.RCARCA是一種核編碼的葉綠體蛋白，具有分子伴侶的功能，在多數(shù)物種中含有α和β兩種亞基。?Inmaize,twoβ-

2、isoformRCAgenes(Zmrca1andZmrca2)withanidenticalopenreadingframe(ORF)werereported.翻譯43kD（β亞基）水解41kDVargas-Suarezetal.,JExpBot,2004Ayala-Ochoaetal.,Biochimie2004玉米在熱脅迫條件下產(chǎn)生45-46kDRCA多肽ZoranRisticetal.JExpBot,2009Questions1玉米中是否存在RCAα亞基和基因？2前人報道的45-46kDRCA多肽來源？3玉米RCA

3、基因與產(chǎn)量的關(guān)系如何?RESULTS1.GenomicAnalysisandcDNACloningRevealedaNewα-formRCAGeneandaPreviouslyReportedβ-formGene2.Zmrca1andZmrca3EncodedtwoDifferentMaizePolypeptidePanelA:anti-Zmrca3antibody.PanelB:anti-Zmrca1antibody.Lane1:pET-30a-Zmrca1PanelC:nativeZmrca1(lane1),lane2

4、:pET-30a-Zmrca3nativeZmrca3(lane2),lane3:emptyE.colileafprotein(lanes3,4)lane4:E.colitransformedwithemptypET-30alane5:Arabidopsislane6:maize.RecombinantNativeZmrca3Zmrca3RecombinantNativeZmrca1Zmrca1MALDI-TOF-MSanalysis3.Zmrca1andZmrca3WereTargetedtoChloroplast4.Z

5、mrca1andZmrca3ShowedLeaf-predominantandCyclicDay/NightExpressionPatterns5.TheExpressionLevelsoftheZmrca1andZmrca3WereCorrelatedWithGrainYieldinMaizeMarkerlociassociatedwithgeneexpressionandgrainyieldin123maizeinbredlinesCONCLUSION?Inmaize,anewα-isoformgeneZmrca3fu

6、nctionsalongsideapreviouslyreportedβ-isoformgeneZmrca1.?TheZmrca3encodesalargermaizeRCApolypeptide,anditsproteinproductislocalizedinchloroplasts.?SimilartotheZmrca1,theZmrca3transcriptsaccumulatemoreintheleafthaninothertissuesandshowcyclicvariationsduringaday/nigh

7、tperiod.?BothZmrca1andZmrca3playanimportantroleindetermininggrainyieldandmayhavepotentialuseinbreedingapplicationstoenhancemaizeproductivity.Yinetal.,(2013)Anα-isoformgenefunctionsalongsideaβ-isoformgeneinmaize,submittedtoPlantPhysiology.Inrevision近年來以第1作者身份發(fā)表的SCI

8、文章1)ExpressionquantitativetraitlocianalysisoftwogenesencodingRubiscoactivaseinsoybean.PlantPhysiology,2010，152:1625-1637（IF>6)2)Mappingofquantitativetra