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《轉(zhuǎn)磷酸甘露糖變位酶基因提高水稻維生素c含量》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、中國水稻科學(xué)(ChinJRiceSci),２０１６,３０(４):４４１－４４６http://www．ricesci．cnDOI:１０．１６８１９/j．１００１Ｇ７２１６．２０１６．５１２９４４１轉(zhuǎn)磷酸甘露糖變位酶基因提高水稻維生素C含量高利芬１,２夏志輝２,３張繼１王道文２翟文學(xué)２,?(１江漢大學(xué)系統(tǒng)生物學(xué)研究院,武漢４３００５６;２中國科學(xué)院遺傳與發(fā)育生物學(xué)研究所,北京１００１０１;３海南大學(xué)生命科學(xué)研究院,?？冢担罚埃玻玻??通訊聯(lián)系人,EＧmail:wxzhai＠genetics．a(chǎn)c．cn)Transgenosisofth
2、ePhosphomannomutaseTransgeneIncreasesVitaminCContentinRiceGAOLiＧfen１,２,XIAZhiＧhui２,３,ZHANGJi１,WANGDaoＧwen２,ZHAIWenＧxue２,?(１InstituteforSystemsBiology,JianghanUniversity,Wuhan４３００５６,China;２InstituteofGeneticsandDevelopmentalBiology,ChineseAcademyofSciences,Beijing１００１０
3、１,China;３InstituteofLifeSciences,HainanUniversity,Haikou５７０２２８,China;?Correspondingauthor,EＧmail:wxzhai＠genetics．a(chǎn)cc．n)GAOLifen,XIAZhihui,ZHANGJi,etal．TransgenosisofthephosphomannomutasetransgeneincreasesvitaminCcontentinrice．ChinJRiceSci,２０１６,３０(４):４４１Ｇ４４６．Abstract:V
4、C(vitaminC)isanessentialnutrienttohumanhealth．DuetolackofLＧgulonolactoneoxidase,thelastenzymeinvolvedinVCsynthesispathway,humancouldnotsynthesizeVCbythemselves．RiceisanimportantfoodcropanditsnutritionalvaluecouldbegreatlyimprovedbyincreasingtheVCcontentinriceseeds．Pho
5、sphomannomutase(PMM)isanimportantenzymeinVCsynthesispathway,catalyzingtheinterconversionfrommannose６Ｇphosphatefrommannose１Ｇphosphate．Inthisstudy,OryzaPMMgene(OsPMM)thatwasunderthecontrolofseedＧspecificexpressedpromoterBx１４wastransferredinto‘C４１８’,arestorerlineofthreeＧ
6、linejaponicahybridrice,usingthedoublerightＧbordervectorpMNDRBBin６throughanAgrobacteriumtumefaciensＧmediatedsystem．MolecularanalysisrevealedthatOsPMMwasintegratedintothegenomeoftransgenic‘C４１８’,andthehomozygousandmarkerＧfreetransgeniclinewasobtainedintheT２generation．Ge
7、neexpressionanalysisoftransgeniclinesshowedtheexpressionlevelofOsPMMwassignificantlyincreasedinseeds,andaccordingly,theVCcontentintheseedsoftransgenicplantalsoincreasedby２５－５０％．Keywords:vitaminC;phosphomannomutase;doublerightＧbordervectorsystem;markerＧfree;transgenicl
8、ine高利芬,夏志輝,張繼,等．轉(zhuǎn)磷酸甘露糖變位酶基因提高水稻維生素C含量．中國水稻科學(xué),２０１６,３０(４):４４１Ｇ４４６．摘要:維生素C(VC)是人體健康所必需的營養(yǎng)元素.人類由于缺乏VC合成途徑中的最后一種酶(LＧ古洛糖酸內(nèi)酯氧化酶),自身
