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1、纖細(xì)席藻對(duì)重金屬鎳脅迫的響應(yīng)研究陳蘭洲汪靜武艷芳柯檀梁亞楠?jiǎng)⑺棘幬錆h大學(xué)資源與環(huán)境科學(xué)學(xué)院湖北省環(huán)境修復(fù)技術(shù)研究中心武漢文科生態(tài)環(huán)境有限公司實(shí)驗(yàn)研宄丫纖細(xì)席藻Phormidiumtcnuc對(duì)重念屬線脅迫的光合生理響應(yīng)及外源抗氧化劑的保護(hù)作用,結(jié)果發(fā)現(xiàn):在不同濃度Ni2+短期處理纖細(xì)席藻時(shí),其熒光產(chǎn)量隨處理時(shí)間先發(fā)生急劇下降,隨后有所回升;熒光產(chǎn)量隨著Ni2+濃度的增加而明顯降低,在100mg/LNi21濃度下熒光曲線的0、J、I、P相完全消失,光合活性(Fv/Fm)喪失.經(jīng)抗氧化劑抗壞血酸(ASC)和N-乙酰半胱氨酸(N
2、AC)分別預(yù)處理的纖細(xì)席藻熒光產(chǎn)量有一定程度恢復(fù),且ASC抗氧化作用相比NAC更加明顯,但其保護(hù)作用均隨著時(shí)間增加而逐漸減弱.在不同濃度的Ni2+長(zhǎng)期脅迫時(shí),Id內(nèi)抑制作用顯著,葉綠素a、光合活性及電子傳遞鏈光合參數(shù)(ET0/ABS、ETO/TRO、RC/CS0)下降顯著,SOD活性升高.實(shí)驗(yàn)結(jié)果表明:鎳對(duì)纖細(xì)席藻的光合生理活性具有較強(qiáng)的抑制作用,而外源抗氧化劑可以短期內(nèi)有效緩解重金屬鎳脅迫造成的損傷,因此在吸附去除重金屬時(shí)可有效提高藻類的吸附效率和循環(huán)利用.關(guān)鍵詞:纖細(xì)席藻;鎳;葉綠素?zé)晒鈩?dòng)態(tài);抗氧化系統(tǒng);陳蘭洲(19
3、76-),男,教授,生導(dǎo)師,,研究方向:環(huán)境生物學(xué),E-mail:chcnlz@whu.edu.cn2017-09-12基金:國(guó)家自然科學(xué)基金資助項(xiàng)目(31370421)TheResponsesofPhormidiumtenueontheStressofHeavyMetalNickelChenLanzhouWangJingWuYanfangKeTanLiangYananLiuSiyaoSchoolofResource&EnvironnientalScience,HubeiResearchCenterofEnvironme
4、ntRemediationTechnology,WuhanUniversity;WuhanWenkeEcologicalEnvironmentLtd.;Abstract:TheeffectsofnickelonPhormidiumtenue,includingphotosyntheticactivity,antioxidantenzymesandcarbohydratemetabolismwereinvestigated.Itwasfoundthatfluorescenceintensitydecreasedrapidl
5、yinthefirst10minafterNi2.wasaddedintothesystem,andpartlyrecoveredtothenormal.icontentscouldinhibitthefluorescenceproductionofP-tenueandaggravatedastheincreaseofNi"concentration,evenO-I-J-PphasesofchlorophyllafluorescencetransientsdisappearedabsolutelyaswellasFv/
6、Fniwith100mg/LNi21.Thetwoantioxidants,ASCandNAC,wereabletorelievethestressofNi2onP.tenue,andASCwasbetteratprotectingcapacity,whiletheanti-oxidationeffectsdecreasedasextensionoftreatmenttime.Thechlorophyllacontent,Fv/Fmandphotosyntheticparameters(ET0/ABS、ET0/TK0、R
7、C/CS0)decreasedsignificantlyafterNi2.stressingfor24h,meanwhileSODactivityoppositelyincreasedtodefendagainststressofNi2..TheresultsindicatedthatNi2+hadaninhibitoryeffectonphotosynthesisofP.tenue,andantioxidantscouldrelieveeffectivelytheinhibition,whichcouldincreas
8、etheabsorptionefficiencyandrecyclingutilizationofalgaeasbio-adsorbents.Keyword:Phormidiumtenue;Ni;chlorophyllafluorescencetransients;antioxygensystem;Received: