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1、aB—crystallin在喉癌組織中的表達及臨床意義［摘要］目的探討c(B-crystallin基因在喉癌組織中的表達及其與喉癌的關系。方法收集2008年3月?2014年10月南京醫(yī)科大學第二附屬醫(yī)院及皖南醫(yī)學院弋磯山醫(yī)院手術切除的喉癌標本54例及13例癌旁正常黏膜標本，采用實時熒光定量PCR(Real-timePCR)檢測8例喉癌組織及其癌旁正常組織中aB-crystallin基因的表達，免疫組化法檢測54例癌組織及13例癌旁正常組織中ctB-crystallin蛋白的表達，比較不同組織中ctB-crystallin陽性表達率。結(jié)果喉癌組織
2、中的aB-crystallinmRNA表達量及aB-crystallin蛋白陽性表達率均明顯高于癌旁正常組織，差異均有高度統(tǒng)計學意義(P0.05)。結(jié)論aB-crystallin基因的高表達與喉癌發(fā)生發(fā)展及轉(zhuǎn)移有關，可能為喉癌患者的預后提供一個潛在的風險評估指標。［關鍵詞］aB-crystallin；實時熒光定量PCR;喉癌；免疫組化［中圖分類號］R739.63［文獻標識碼］A［文章編號］1673-7210(2016)10(a)-0009-04ExpressionandsignificanceofaB-crystallininlaryngeal
3、squamouscellcarcinomaXIONGLinlMAOYuan2MAJun3ZHANGDawei4AZHUJin5CHENRenjie41.DepartmentofPathology，theSecondAffiliatedHospitalofNanjingMedicalUniversity，JiangsuProvince，Nanjing210029，China；2.DepartmentofOtolaryngology-HeadandNeckSurgery，JiangsuProvincialOfficialHospital，Jiang
4、suProvince，Nanjing210024，China；3.DepartmentofOtolaryngology-HeadandNeckSurgery，YijiShanHospitalofWannanMedicalCollege，AnhuiProvince，Wuhu241001，China；4.DepartmentofOtolaryngology-HeadandNeckSurgery,theSecondAffiliatedHospitalofNanjingMedicalUniversity,JiangsuProvince，Nanjing2
5、10029，China；5.MilitaryMedicalResearchInstituteofNanjingMilitaryRegion，JiangsuProvince，Nanjing210002，China[Abstract]ObjectiveToexaminetheexpressionofaB-crystallininhumanlaryngealsquamouscellcarcinoma(LSCC)andinvestigatetherelationshipbetweenitsexpressionandLSCC.MethodsAtotalo
6、f54casesofLSCCand13casesofadjacentnormaltissueswerecollectedfromtheSecondAffiliatedHospitalofNanjingMedicalUniversityandYijiShanHospitalofWannanMedicalCollegefromMarch2008toOctober2014.Real-timequantitativepolymerasechainreaction(8LSCCtissues，8adjacentnormaltissues)andimmuno
7、histochemistry(54LSCCtissuesand13adjacentnormaltissues)wereperformedtodetectedtheexpressionofaB-crystallingeneandproteininLSCC.ThepositiveexpressionrateofaB-crystallinindifferenttissueswascompared.ResultsThepositiveexpressionrateofaB-crystallinmRNAandproteininLSCCweresignifi
8、cantlyhigherthanthoseintumor-adjacentnormaltissues,thedifferenceswerestatis
