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1、aB—crystallin在喉癌組織中的表達(dá)及臨床意義［摘要］目的探討c(B-crystallin基因在喉癌組織中的表達(dá)及其與喉癌的關(guān)系。方法收集2008年3月?2014年10月南京醫(yī)科大學(xué)第二附屬醫(yī)院及皖南醫(yī)學(xué)院弋磯山醫(yī)院手術(shù)切除的喉癌標(biāo)本54例及13例癌旁正常黏膜標(biāo)本，采用實(shí)時(shí)熒光定量PCR(Real-timePCR)檢測(cè)8例喉癌組織及其癌旁正常組織中aB-crystallin基因的表達(dá)，免疫組化法檢測(cè)54例癌組織及13例癌旁正常組織中ctB-crystallin蛋白的表達(dá)，比較不同組織中ctB-crystallin陽性表達(dá)率。結(jié)果喉癌組織
2、中的aB-crystallinmRNA表達(dá)量及aB-crystallin蛋白陽性表達(dá)率均明顯高于癌旁正常組織，差異均有高度統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論aB-crystallin基因的高表達(dá)與喉癌發(fā)生發(fā)展及轉(zhuǎn)移有關(guān)，可能為喉癌患者的預(yù)后提供一個(gè)潛在的風(fēng)險(xiǎn)評(píng)估指標(biāo)。［關(guān)鍵詞］aB-crystallin；實(shí)時(shí)熒光定量PCR;喉癌；免疫組化［中圖分類號(hào)］R739.63［文獻(xiàn)標(biāo)識(shí)碼］A［文章編號(hào)］1673-7210(2016)10(a)-0009-04ExpressionandsignificanceofaB-crystallininlaryngeal
3、squamouscellcarcinomaXIONGLinlMAOYuan2MAJun3ZHANGDawei4AZHUJin5CHENRenjie41.DepartmentofPathology，theSecondAffiliatedHospitalofNanjingMedicalUniversity，JiangsuProvince，Nanjing210029，China；2.DepartmentofOtolaryngology-HeadandNeckSurgery，JiangsuProvincialOfficialHospital，Jiang
4、suProvince，Nanjing210024，China；3.DepartmentofOtolaryngology-HeadandNeckSurgery，YijiShanHospitalofWannanMedicalCollege，AnhuiProvince，Wuhu241001，China；4.DepartmentofOtolaryngology-HeadandNeckSurgery,theSecondAffiliatedHospitalofNanjingMedicalUniversity,JiangsuProvince，Nanjing2
5、10029，China；5.MilitaryMedicalResearchInstituteofNanjingMilitaryRegion，JiangsuProvince，Nanjing210002，China[Abstract]ObjectiveToexaminetheexpressionofaB-crystallininhumanlaryngealsquamouscellcarcinoma(LSCC)andinvestigatetherelationshipbetweenitsexpressionandLSCC.MethodsAtotalo
6、f54casesofLSCCand13casesofadjacentnormaltissueswerecollectedfromtheSecondAffiliatedHospitalofNanjingMedicalUniversityandYijiShanHospitalofWannanMedicalCollegefromMarch2008toOctober2014.Real-timequantitativepolymerasechainreaction(8LSCCtissues，8adjacentnormaltissues)andimmuno
7、histochemistry(54LSCCtissuesand13adjacentnormaltissues)wereperformedtodetectedtheexpressionofaB-crystallingeneandproteininLSCC.ThepositiveexpressionrateofaB-crystallinindifferenttissueswascompared.ResultsThepositiveexpressionrateofaB-crystallinmRNAandproteininLSCCweresignifi
8、cantlyhigherthanthoseintumor-adjacentnormaltissues,thedifferenceswerestatis
