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1、生物秀實驗頻道——生命科學(xué)實驗中心www.bbioo.com/bio101SouSoutthheerrnn,,NNoortrthheerrnnaandndWesWestteerrnnbblloottttiingng生理所黃阿敏生物秀—專心做生物！www.bbioo.com易生物-領(lǐng)先的生物醫(yī)藥商務(wù)平臺www.ebioe.com生物秀論壇-學(xué)術(shù)交流,資源共享,互助社區(qū)www.bbioo.com/bbs/生物秀論壇——學(xué)術(shù)交流、資源共享、互助社區(qū)www.bbioo.com/bbs生物秀實驗頻道——生命科學(xué)實驗中心www.bbioo.com/bio101Compari
2、sonofSouthern,Northern,andWesternanalysesofGeneX生物秀論壇——學(xué)術(shù)交流、資源共享、互助社區(qū)www.bbioo.com/bbs生物秀實驗頻道——生命科學(xué)實驗中心www.bbioo.com/bio101SouthernhybridizationnFirstdescribedbyE.M.Southernin1975.nApplicationsofSouthernhybridizationnRFLP’s,VNTR’sandDNAfingerprintingnCheckingofthegeneknockoutmicenThe
3、flowchartofSouthernhybridization生物秀論壇——學(xué)術(shù)交流、資源共享、互助社區(qū)www.bbioo.com/bbs生物秀實驗頻道——生命科學(xué)實驗中心www.bbioo.com/bio101SouthernhybridizationTransferbuffer生物秀論壇——學(xué)術(shù)交流、資源共享、互助社區(qū)www.bbioo.com/bbs生物秀實驗頻道——生命科學(xué)實驗中心www.bbioo.com/bio101DetectionofanRFLPbySouthernblotting生物秀論壇——學(xué)術(shù)交流、資源共享、互助社區(qū)www.bbioo.c
4、om/bbs生物秀實驗頻道——生命科學(xué)實驗中心www.bbioo.com/bio101Detectionofthesickle-cellglobingenebySouthernblotting生物秀論壇——學(xué)術(shù)交流、資源共享、互助社區(qū)www.bbioo.com/bbs生物秀實驗頻道——生命科學(xué)實驗中心www.bbioo.com/bio101Morethan3000humangeneticdiseasesareattributabletosingle-genedefects.Inmostofthesethemutationisrecessive:thatis,it
5、showsitseffectonlywhenanindividualinheritstwodefectivecopiesofthegene,onefromeachparent.Onegoalofmodernmedicineistoidentifythosefetusesthatcarrytwocopiesofthedefectivegenelongbeforebirthsothatthemother,ifshewishes,canhavethepregnancyterminated.Insickle-cellanemia,forexample,theexactn
6、ucleotidechangeinthemutantgeneisknown(thesequenceGAGischangedtoGTGataspecificpointintheDNAstrandthatcodesfortheβchainofhemoglobin).Forprenataldiagnosis,twoDNAoligonucleotidesaresynthesized-onecorrespondingtothenormalgenesequenceintheregionofthemutationandtheothercorrespondingtothemut
7、antsequence.Iftheoligonucleotidesarekeptshort(about20nucleotides),theycanbehybridizedwithDNAatatemperatureselectedsothatonlytheperfectlymatchedhelixwillbestable.SucholigonucleotidescanthusbeusedaslabeledprobestodistinguishbetweenthetwoformsofthegenebySouthernblottingonDNAisolatedfrom
8、fetalcellsco
