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1、Microbiol.Immunol.,40(6),425-433,1996ComparisonoftheAminoAcidSequenceandPhylogeneticAnalysisofthePeplomer,IntegralMembraneandNucleocapsidProteinsofFeline,CanineandPorcineCoronavirusesKenjiMotokawa1,TsutomuHohdatsu*1,HiroshiHashimoto2,andHiroyukiKoyama11DepartmentofVeterinaryInf
2、ectiousDiseases,SchoolofVeterinaryMedicineandAnimalSciences,KitasatoUniversity,Towada,Aomori034,Japan,and2DepartmentofVirology,CenterofBasicResearch,TheKitasatoInstitute,Minato-ku,Tokyo108,JapanReceivedOctober13,1995;inrevisedform,February19,1996.AcceptedMarch1,1996Abstract:Com
3、pletenucleotidesequencesweredeterminedbycDNAcloningofpeplomer(S),integralmem-brane(M)andnucleocapsid(N)genesoffelineinfectiousperitonitisvirus(FIPV)typeIstrainKU-2,UCD1andBlack,andfelineentericcoronavirus(FECV)typeIIstrain79-1683.OnlyMandNgeneswereanalyzedinstrainKU-2andstrain7
4、9-1683,whichstillhadunknownnucleotidesequences.DeducedaminoacidsequencesofS,MandNproteinswerecomparedinatotalof7strainsofcoronaviruses,whichincludedFIPVtypeIIstrain79-1146,caninecoronavirus(CCV)strainInsavc-1andtransmissiblegastroenteritisvirusofswine(TGEV)strainPurdue.Comparis
5、onofdeducedaminoacidsequencesofMandNproteinsrevealedthatbothMandNproteinshadanidentityofatleast90%betweenFIPVtypeIandtypeII.Thephylo-genetictreeoftheMandNprotein-deducedaminoacidsequencesshowedthatFIPVtypeIandtypeIIformagroupwithFECVtypeII,andthattheseviruseswereevolutionarilyd
6、istantfromCCVandTGEV.Ontheotherhand,whentheSprotein-deducedaminoacidsequenceswascompared,identityofonlyabout45%wasfoundbetweenFIPVtypeIandtypeII.ThephylogenetictreeoftheSprotein-deducedaminoacidsequencesindicatedthatthreestrainsofFIPVtypeIformagroup,andthatitisaverylongdistance
7、fromtheFIPVtypeII,FECVtypeII,CCVandTGEVgroups.Keywords:Felineinfectiousperitonitisvirus,Felineentericcoronavirus,AminoacidsequenceFelineinfectiousperitonitisvirus(FIPV),familytypesofFIPV,andtheantigenicityoftypeIIstrainofCoronaviridae,genusCoronavirus,causesachronic,FIPVandFECV
8、wereclosertoTGEVandCCVthantoprogressive,immunologicall
