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1、中國水稻科學(xué)(ChinJRiceSci),２０１４,２８(２):１２５－１３１http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１４．０２．００３１２５水稻千粒重QTLqTGW１．１的驗證張宏偉朱玉君陳玉宇陳俊宇黃得潤莊杰云?(中國水稻研究所國家水稻改良中心/水稻生物學(xué)國家重點實驗室,杭州３１０００６;?通訊聯(lián)系人,EＧmail:jz１８０３＠hzcnc．com)ValidationofqTGW１．１,aQuantitativeTraitLocusfor１０００ＧgrainWeightinRice(Oryzasat
2、ivaL．)ZHANGHongＧwei,ZHUYuＧjun,CHENYuＧyu,CHENJunＧyu,HUANGDeＧrun,ZHUANGJieＧyun?(ChineseNationalCenterforRiceImprovement/StateKeyLaboratoryofRiceBiology,ChinaNationalRiceResearchInstitute,Hangzhou３１０００６,China;?Correspondingauthor,EＧmail:jz１８０３＠hzcncc．om)ZHANGHongwei,ZHUYujun,CHENYuyu,etal．
3、ValidationofqTGW１．１,aquantitativetraitlocusfor１０００Ｇgrainweightinrice(OryzasativaL．)．ChinJRiceSci,２０１４,２８(２):１２５Ｇ１３１．Abstract:Thisstudywasconductedtovalidateaquantitativetraitlocus(QTL)for１０００Ｇgrainweight(TGW)whichwaspreviouslymappedina３．７Mbregiononthelongarmofricechromosome１,andtolocate
4、thisQTLmoreprecisely．AplantcarryingaheterozygoussegmentextendingfromRM１１４４８toRM１１６１５wasselectedfromthe３/Milyang４６BCZhenshan９７２F５population．TheresultingBC２F６andBC２F７populationswereassayedwithSSRmarkers．ThreeBC２F７plantswereidentified,carryingheterozygoussegmentscoveringtheintervalsRM１１４４８
5、－RM１１５２２,RM１１４４８－RM１１５４９andRM１２３２－RM１１６１５,respectively．NonＧrecombinanthomozygoteswereidentifiedfromtheBC２F８populations,fromwhichthreesetsofnearisogeniclines(NILs)intheBC２F８∶９generationwereproduced,consistingoftwoparentalgenotypesintheintervalsRM１１４４８－RM１１５２２,RM１１４４８－RM１１５４９andRM１２３２－RM１
6、１６１５,respectively．Fieldtrialswereconductedtomeasureheadingdate(HD),TGW,andgrainshapetraitsincludinggrainlengthandwidth．TwoＧwayanalysisofvariancewasperformedtotestthephenotypicdifferencesbetweentwogenotypicgroupsineachNILsetusingSASprogram．NosignificantvariationswereobservedonTGWandgrain
7、shapeintheNILwhichwasheterogeneousintheintervalRM１１４４８－RM１１５２２,whereassignificantgenotypiceffectsweredetectedintheremainingtwoNILsinwhichtheenhancingalleleswereallderivedfromZhenshan９７．ForHD,nosignificanteffectsweredetectedinthethreeNILs．Basedoncomparisonamonggenomiclocationsof
