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1、宜賓職業(yè)技術學院論文α-淀粉酶的生產(chǎn)工藝系部生物與化工工程系專業(yè)名稱釀酒技術班級釀酒11103姓名王夢韻李艷梅嚴紅俊學號201117315201117404201117346指導教師蘭小艷二〇一二年十月二十九日α-淀粉酶的生產(chǎn)工藝摘要α-淀粉酶廣泛分布于動物、植物和微生物中,能水解淀粉產(chǎn)生糊精、麥芽糖、低聚糖和葡萄糖等,是工業(yè)生產(chǎn)中應用最為廣泛的酶制劑之一。目前,α-淀粉酶已廣泛應用于變性淀粉及淀粉糖、焙烤工業(yè)、啤酒釀造、酒精工業(yè)、發(fā)酵以及紡織等許多行業(yè)。本次設計的淀粉酶發(fā)酵,分別以玉米粉為碳源,以豆餅為氮源,以BF-7658枯草芽孢桿
2、菌為生產(chǎn)菌種,同時做出了生產(chǎn)工藝流程圖,詳細的介紹了α-淀粉酶的生產(chǎn)工藝。關鍵詞:α-淀粉酶;生產(chǎn)工藝設計;深層發(fā)酵法AlphaamylaseproductiontechnologyAbstractAlphaamylasewidelydistributedinanimals,plantsandmicrobes,canbehydrolyzedstarchproducedextrin,maltose,oligosaccharideandglucoseandsoon,itistheindustrialproductioninthemostwi
3、delyusedoneofenzymepreparation.Atpresent,thealphaamylasehasbeenwidelyusedinmodifiedstarchandstarchsugar,bakingindustry,brewing,alcoholindustry,hairleavenandtextileandmanyotherindustries.Thedesignofamylasefermentation,respectivelywithcornflourforcarbonsource,nitrogensourc
4、eforbeancake,withBF-7658bacillussubtilisstrainsfortheproduction,atthesametime,maketheproductionprocessflowdiagram,detailedintroducesthealphaamylaseproductiontechnology.Keywords:Alphaamylase,Productionprocessdesign;Deepfermentation目錄前言.....................................
5、...........................................................................................11α-淀粉酶的生產(chǎn)方法..................................................................................................11.1生產(chǎn)方法的選擇...........................................................
6、........................................11.2α-淀粉酶培養(yǎng)基的制作....................................................................31.2.1耐高溫α-淀粉酶的生產(chǎn)方法............................................................................41.2.2耐高溫α-淀粉酶的生產(chǎn)原料..................................
7、.......................................41.2.3耐高溫α-淀粉酶培養(yǎng)基制作.........................................................................51.2.4耐高溫α-淀粉酶的另一種生產(chǎn)方法.............................................................51.2.5用菌種枯草芽孢桿菌來提取耐高溫α-淀粉酶...............................
8、..............52α-淀粉酶的菌種選育......................................................................62.1菌株與