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1、T淋巴細胞在小鼠腎缺血再灌注損傷中的作用2O07年2月第15卷第1期中國實驗動物ACTALABOBATOBIUMANIMALISSCIENTIASINICAFeb.,V01.152Oo7No.1,e)研究報告55蝗七,,蛉e,T淋巴細胞在小鼠腎缺血再灌注損傷中的作用陳恕求,陳明,張古田,李俊龍,彭濤,孫則禹(南京大學醫(yī)學院附屬鼓樓醫(yī)院泌尿外科,南京210008)【摘要】目的研究T淋巴細胞在腎缺血再灌注損傷(ira)導致的急性腎損害中的作用.方法BALB/c小鼠和BALB/c裸小鼠各24只,分別隨機分為A.一.組和
2、B.一.組,每組6只.雙腎蒂阻斷45min后恢復血流建立腎IRI模型,假手術對照組,lRI后24,48和72h時檢測scr,尿蛋白定量及腎病理學,A組檢測脾T細胞亞群;對比BALB/c小鼠和BALB/c裸小鼠的腎功能下降,組織學損害程度以及脾T淋巴細胞亞群變化.結果A2一.組和B2一.組均有Scr和尿蛋白定量明顯升高(P<0.05),且A組損害程度明顯重于B組(P<0.05);A2一.組出現(xiàn)典型的IRI組織損害表現(xiàn)(P<0.05),B2一.組無明顯IRI組織損害(P>0.05);A2—3組
3、脾CD3'T細胞百分比較A.組升高(P<0.05),而CD4,CD8比值無明顯變化(P>0.05).結論T淋巴細胞是小鼠腎IRI導致急性腎損害的重要病理生理學因素.【關鍵詞】T淋巴細胞;再灌注損傷;腎;小鼠【中圖分類號】R318.06【文獻標識碼】A【文章編號】1005.4847(2007)O1.0021.04PathophysiologicRoleofTLymphocytesinRenallschemia—ReperfusionInjuryinMiceCHENShu-qiu,CHENMing,ZHA
4、NGGu?tian,LIJun-long,PENGTao,SUNZe?yu(DepartmentofUrology,TheAffiliatedDrumTowerHospitalofNanjingUniversityMedicalSchool,Nanjing210008,China)【Abstract】ObjectiveToinvestigatethepathophysiologicroleofTlymphocytesinrenalischemia-reperfusioninjuryinmice.MethodsBA
5、LB/cwild?mice(groupA,n=24)andBALB/cnude?mice(groupB,n=24)weredividedintofourgroups(n=6),respectively,thegroupsAltoA4andgroupsBltoB4.Bilateralrenalpedicleswereoccludedbymicrovascularclampsfor45minutes.Thenthekidneyswerereperfused.Sampleswerecollectedatshamoper
6、ationand24h,48hand72hafterIBI.Serumcreatinine(Scr),urineproteinconcentration,renalhistologyandsplenicCD3/CD4/CD8positivecells(groupA)wereexamined,respectively.ResultsScrandurineproteinconcentrationincreasedandhistologicalinjuryWasobservedingroupA2—4andB2—4.Th
7、einjuryingroupAweremoreseverethanthatingroupB(P<0.05).FACSanalysisshowed,thatincomparisonwithgroupAl,percentageofsplenicCD3TcellsingroupA2andA3increasedsignificantly(P<0.05),butasimilarratioofCIM/CD8'betweengroupAIandgroupA2—3(P夕0.05)).ConclusionTlympho
8、cytesplayanimportantpathophysiologicroleinrenalischemia?reperfusioninjury,andmaymediatepost?ischemicdeclineinrenalfunctionandleadto~stologicalinjury.【Keywords】Tlymphocytes;Reperfusioninju
