資源描述:
《園藝專(zhuān)業(yè)畢業(yè)生優(yōu)秀論文-不同鮮食葡萄品種花粉生活力測(cè)定及貯藏特性研究》由會(huì)員上傳分享,免費(fèi)在線(xiàn)閱讀,更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、不同鮮食葡萄品種花粉生活力測(cè)定及貯藏特性研究作者劉君麗指導(dǎo)教師李桂榮(河南科技學(xué)院園藝園林學(xué)院,河南新鄉(xiāng)453003)摘要:[目的]花粉生活力的高低直接影響葡萄雜交育種效率的高低或成敗??焖贉?zhǔn)確測(cè)定不同鮮食葡萄品種的花粉生活力,可為正確選擇雜交組合和授粉時(shí)間等提供參考依據(jù),也是保證雜交育種順利進(jìn)行的關(guān)鍵。[方法]以新鮮葡萄花粉為試材,用離體培養(yǎng)法測(cè)定花粉生活力,并探討不同貯藏條件對(duì)鮮食葡萄花粉萌發(fā)的影響。[結(jié)果]通過(guò)鏡檢發(fā)現(xiàn)基拉爾花粉敗育;不同品種的花粉生活力存在差異,自然陰干條件下供試品種花粉的生活力高低順序?yàn)椋亨嵵菰缂t>芳香拉查基>瑞比爾>澤玉>京秀,其花粉生活力分別為50.41%、46
2、.22%、43.04%、40.35%和24.13%,京秀與其它4個(gè)品種差異極顯著。在不同的貯藏條件下,花粉生活力隨著貯藏時(shí)間的延長(zhǎng)而下降,不同的貯藏條件對(duì)其有很大的影響,適當(dāng)?shù)牡蜏乜捎行а娱L(zhǎng)花粉貯藏時(shí)間。其中-40℃萌發(fā)率最高,為最佳貯藏方法;在貯藏過(guò)程中,澤玉和京秀存在“短期被迫休眠現(xiàn)象”。關(guān)鍵字:鮮食葡萄,花粉,生活力,貯藏StoragelifeandPollenViabilityTestofDifferenttablegrapeCultivarsAuthorLiuJun-liSupervisorLiGui-rong(SchoolofHorticultureandLandscapeArc
3、hitecture,HenanInstituteofScienceandTechnology,XinxiangHenan453003)Abstract:[0bjeetive]Theefficiencyandthesuccessorfailureofhybridizationforgrapewereinfluencedbypollen’sgerminationability.Hence,themethodsformeasuregrapepollen’sgerminationabilitywhichisquicklyandaccuratecouldprovidereferencesforcros
4、scombinationandpollinationtime.a(chǎn)lsoitisthekeyforsuccessofbridization.[Method]Withthefreshpollensofgrapeasthetestedmaterials,determinationofpollenviabilitybytestingmethodofpollensculturedinvitro,andtheeffectsofdifferentstorageconditiononthepollengerminationoftable-grapewerealsodiscussed.[Result]Itwa
5、sfoundthatkielarpollenabortionassayedundermicroscope.thepollenviabilityofdifferentofvarietiesaredifferent.Naturaldryingconditionstestedviabilityofpollenoftheorderfromhightolowis:zhengzhouzaohong>芳香拉查基>riber>zeyu>jingxiu,Thepollenviabilitywere50.41%,46.22%,43.04%,40.35%and24.13%.jingxiuandtheother4v
6、arietiesshowedsignificantdifference.Underdifferentstorageconditions,vitalityofpollenweregraduallydescendaslastofstoretime,differentstorageconditionshaveaimpactonits,thesuitablelowtemperatureeffectiveextensionthestoretimeofpollen.Thebestmethodstokeepvitalityofpollenat-40℃,thegerminationofpollenishig
7、her;duringprocessofstorage,theappearanceof“compelleddormancyofshort-term”wasexistofzeyuandjingxiu.Keywords:table-grape,pollen,viability,storage1引言葡萄(Grapes),又名蒲桃、草龍珠、山葫蘆、李桃,為葡萄科(VitaceaeLindley)葡萄屬(VitisL.)