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1、標(biāo)準(zhǔn)緩沖液pH值與溫度對(duì)照表溫度℃四草酸氫鉀0.05M鄰苯二甲酸氫鉀0.05M混合磷酸鹽0.025M硼砂0.01M51.674.006.959.39101.674.006.029.33151.674.006.909.28201.684.006.889.23251.684.006.869.18301.684.016.859.14351.694.026.849.11常用緩沖溶液的配制方法1．甘氨酸–鹽酸緩沖液（0.05mol/L）X毫升0.2mol/L甘氨酸+Y毫升0.2mol/LHCI，再加水稀釋至200毫升pHXYpHXY2.02.42.62.8505050504
2、4.032.424.216.83.03.23.43.65050505011.48.26.45.0甘氨酸分子量=75.07，0.2mol/L甘氨酸溶液含15.01克/升。?2．鄰苯二甲酸–鹽酸緩沖液（0.05mol/L）X毫升0.2mol/L鄰苯二甲酸氫鉀+0.2mol/LHCl，再加水稀釋到20毫升pH(20℃)XYpH(20℃)XY2.22.42.62.83.0555554.0703.9603.2952.6422.0223.23.43.63.855551.4700.9900.5970.263鄰苯二甲酸氫鉀分子量=204.23，0.2mol/L鄰苯二甲酸氫溶液含4
3、0.85克/升?3．磷酸氫二鈉–檸檬酸緩沖液pH0.2mol/LNa2HPO4（毫升）0.1mol/L檸檬酸（毫升）pH0.2mol/LNa2HPO4（毫升）0.1mol/L檸檬酸（毫升）2.22.42.62.83.03.23.43.63.84.04.24.44.64.85.00.401.242.183.174.114.945.706.447.107.718.288.829.359.8610.3010.6018.7617.8216.8315.8915.0614.3013.5612.9012.2911.7211.1810.6510.149.705.25.45.65.
4、86.06.26.46.66.87.07.27.47.67.88.010.7211.1511.6012.0912.6313.2213.8514.5515.4516.4717.3918.1718.7319.1519.459.288.858.407.917.376.786.155.454.553.532.611.831.270.850.55Na2HPO4分子量=14.98，0.2mol/L溶液為28.40克/升。Na2HPO4-2H2O分子量=178.05，0.2mol/L溶液含35.01克/升。C4H2O7·H2O分子量=210.14，0.1mol/L溶液為21.0
5、1克/升。4．檸檬酸–氫氧化鈉-鹽酸緩沖液pH鈉離子濃度（mol/L）檸檬酸（克）C6H8O7·H2O氫氧化鈉(克)NaOH97%鹽酸（毫升）HCl（濃）最終體積（升）①2.23.13.34.35.35.86.50.200.200.200.200.350.450.3821021021021024528526684838383144186156160116106456810512610101010101010①????使用時(shí)可以每升中加入1克克酚，若最后pH值有變化，再用少量50%?氫氧化鈉溶液或濃鹽酸調(diào)節(jié)，冰箱保存。②?????5．檸檬酸–檸檬酸鈉緩沖液（0.1m
6、ol/L）pH0.1??mol/L檸檬酸（毫升）0.1??mol/L檸檬酸鈉（毫升）pH0.1??mol/L檸檬酸（毫升）0.1??mol/L檸檬酸鈉（毫升）3.03.23.43.63.84.04.24.44.618.617.216.014.914.013.112.311.410.31.42.84.05.16.06.97.78.69.710.85.05.25.45.65.86.06.26.46.68.27.36.45.54.73.82.82.01.411.812.713.614.515.316.217.218.018.64.89.2檸檬酸C6H8O7·H2O：分子
7、量210.14，0.1mol/L溶液為21.01克/升。檸檬酸鈉Na3C6H5O7·2H2O：分子量294.12，0.1mol/L溶液為29.41克/毫升。6．乙酸–乙酸鈉緩沖液（0.2mol/L）pH(18℃)0.2??mol/LNaAc(毫升)0.3??mol/LHAc(毫升)pH(18℃)0.2??mol/LNaAc(毫升)0.3??mol/LHAc(毫升)2.63.84.04.24.44.60.751.201.802.653.704.909.258.808.207.356.305.104.85.05.25.45.65.85.907.007.908.609.
8、109.4
