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1、BMP異位誘導(dǎo)成骨的Micro-CT評(píng)價(jià)作者：李丹 劉民，胡蘊(yùn)玉，畢龍，段永紅，金靜，王軍，劉山金【摘要】借助Micro-CT評(píng)價(jià)單純牛骨形態(tài)發(fā)生蛋白(bovinebonemorphogeneticprotein，bBMP)異位誘導(dǎo)成骨的長(zhǎng)期三維影像學(xué)及骨質(zhì)變化。(20±2)g昆明小鼠21只，麻醉后于雙側(cè)股部肌肉中植入bBMP各2mg，分別于1、2、4、6、8、10、12周各處死3只，切取誘導(dǎo)分化組織，5%戊二醛固定，行Micro-CT掃描和三維重建，運(yùn)用ABA專用骨骼分析軟件測(cè)定組織礦含量(tissuem
2、ineralcontent,TMC)，組織骨密度(tissuemineraldensity,TMD)，骨體積分?jǐn)?shù)(bonevolumefraction,BVF)，結(jié)構(gòu)模型指數(shù)(structuremodelindex,SMI),骨小梁厚度(trabecularthickness,Tb.Th),骨小梁數(shù)量(trabecularnumber,Tb.N)及皮質(zhì)骨骨密度(bonemineraldensity,BMD)等參數(shù)，運(yùn)用SPSS10.0統(tǒng)計(jì)軟件進(jìn)行統(tǒng)計(jì)學(xué)分析。bBMP從植入2周開(kāi)始逐漸形成一橢圓形骨組織塊，2
3、～4周，異位生成骨呈疏松的新生骨，4周時(shí)組織礦含量達(dá)第一個(gè)峰值，骨小梁數(shù)量最多;隨著觀察時(shí)間的延長(zhǎng)(6～12周)，異位誘導(dǎo)生成的橢圓形骨組織內(nèi)部骨小梁逐漸吸收，數(shù)量減少，12周時(shí)骨小梁數(shù)量最少;而外層骨組織逐漸塑形成為皮質(zhì)骨，12周時(shí)骨礦含量值、骨小梁厚度、組織骨密度和皮質(zhì)骨骨密度均達(dá)最大值。說(shuō)明bBMP具有強(qiáng)大的異位骨誘導(dǎo)能力，血供不足時(shí)，骨質(zhì)降解吸收;血供充足時(shí)，骨質(zhì)逐漸成熟改建。12【關(guān)鍵詞】骨形態(tài)發(fā)生蛋白;異位成骨;骨誘導(dǎo);骨小梁;MicroCT Abstract：Toassessthe3-Dim
4、ageandchangesinqualityofosseoustissueproducedthroughectopicosteoinductionbybovinebonemorphegeneticproteinoveranextendedperiodbymeansofMicro-CT.Twenty-oneKunmingrats(20±2)gwereused，2mgbBMPwereimplantedintothighmusclesofbothsidesoftheratunderanesthesia.Three
5、animalsofeachweresacrificedat1,2,4,6,8,10and12weeksaftertheimplantation.Afterbeingharvested,thenewlyformedbonetissuewasfixedwith5%glutaraldehyde,thensuccessivelysubjectedtoMicro-CTscanand3-Dreconstruction,measurementoftissuemineralconsent(TMC)usingABAsoftw
6、areandtissuemineraldensity(TMD),determinationofbonevolumefraction(BVF),structuremodelindex(SMI),trabecularthickness(Tb.Th),trabecularnumber(Tb.N),corticalbonemineraldensity(BMD)andotherparameters,3-DimageswerecreatedandsavedasTIFFfiles;statisticalanalysesw
7、erecarriedoutbyANOVA(analysisofvariance)usingStatisticsPackageforSocialScience(SPSS)software.At2weekspostimplantation,anovalmassofosseoustissuebegantotakeshape;between2to4weeks,the12ectopic-inducednewbonepresentedasloosecancellousbonetissue,andthemineralco
8、ntentreachedthefirstpeakbythe4thweek,showingnumeroustrabeculae;theinnermosttrabecularoftheectopic-inducedlumpofosseoustissuewereresorbedwiththeirnumberdecreasedwithtime(between6thand12thweek),approachingthemi
