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1、萬方數(shù)據(jù)中國組織T程研究與臨床康復(fù)笫f3謄第24嬲2009—06—11出版JournalofClinicalRehabilitativeTissueEngineeringResearchJune11,2009V01.13No.24脂肪干細(xì)胞與軟骨細(xì)胞的共培養(yǎng)☆張勇,趙建寧。韓寧波—__●■l_■■—___—__—●-_●————●—l———●—————__一II一————IIIII●■●一Co-cultureofadiposederivedstemcellsandchondrocytesZhangYong.
2、ZhaoJian-ning,HanNing-bof)eDa巾T櫓ntofOrthopaedics.NanjingGeneraIHospitalofNanjlngMilitaryAreaCommandofChinesePLA,SecondMiIhryMedicalUniversityofChinesePLA.Nanjing210002.JiangsuProvince.ChinaZhangYong☆.&udyingfordoctorate.Attendl叼physjcian.DepartmentofOrthop
3、aedics.NanjingGeneralHospitalofNanjlngMilitaryAreaCommandofChinesepLASecondMilitaryMedicaIUniverailvofChinesePLA.Nanjing210002,JiangsuProvince.ChinaCorrespondenceto:ZhaoJian-ning.Ma8Ier.Professor,DoctoraIsupervisor,DepartmentofOrthopaedics.NanjingGeneralHo
4、spitalofNanjingMilitaryAreaCommandofChinesePLA,SecondMilitaryMedicaIUniversityofChinesePLA.Naniing210002.JiangsuProvince.Chinazhaojianning.0207@163cornReceived:2∞串12.∞^卿惻:2009-02-144632AbstractBACKGROUND:StudyshowsthatbonematToWmesenchyma}stemcellscanbeind
5、ucedintochondrocytesinthemicroenvironmentofarticularcartilage.0BJECTIVE:TovedfYthefeasibilityofinducingadiposededvedstemcells(ADSCs)intochondrocytassoastoprovideaRoveIapproachforarticularcartilagetissueengineering.DESlGN.TIMEANDSETTING:ArandomizedcalItrial
6、wasperformedattheStateKeyLabOratoryofPharrnaceuticaIBiotechnology.NaniingUniversityfromSeptember2007toJuly2008.MATER睢LS:AdultNewZealandwhiterabbitsofcleangrade,weighing1.5Io2.Okg,ofeithergender.METHODS:PrimarvADSCsandaficutarchondrocytesfromNewZealandwhita
7、rabbitswereisolatedandthenseededon6一poreplatesandmillicelIcultureplateinsertsseparately.Aft:er2weeksofco-culture.trypsinizationwasferminatedandADSCsweredetectedwithoiIredOstainingmethod.a(chǎn)lkalinephosphatasedetectionmethodandtoluidinebluestainingmethodrespec
8、tively.MAINOUTCOMEMEASURES:ThecytomorphologyofADSCswasobservedunderinvertedmicroscopebeforeandafterco-culturerespectively.Afterco-culture。theproteoglycanexpressionofADSCswasdetectedwithtoluidinebluestainingme