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《殘耳軟骨細(xì)胞與脂肪干細(xì)胞共培養(yǎng)體內(nèi)構(gòu)建軟骨的實(shí)驗(yàn)研究》由會(huì)員上傳分享,免費(fèi)在線閱讀,更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、萬(wàn)方數(shù)據(jù)組織工程與重建外科雜志2011年4月第7卷第2期doi:10.3969,j.issn.1673—0364.20兒.02.004殘耳軟骨細(xì)胞與脂肪干細(xì)胞共培養(yǎng)體內(nèi)構(gòu)建軟骨的實(shí)驗(yàn)研究張潔蔣海越何樂(lè)仁趙延勇楊慶華韓娟宋宇鵬·75··論著·【摘要l目的驗(yàn)證殘耳軟骨細(xì)胞與脂肪來(lái)源的間充質(zhì)干細(xì)胞(AdiposederivedstemceIls,ADsCs)共培養(yǎng),體內(nèi)構(gòu)建軟骨的可行性。方法分離培養(yǎng)同一先天性小耳畸形患者來(lái)源的殘耳細(xì)胞及脂肪干細(xì)胞。24只裸鼠隨機(jī)分為4組:①實(shí)驗(yàn)組,接種殘耳軟骨細(xì)胞及脂肪干細(xì)胞,兩種細(xì)胞以l:l比例混合,細(xì)胞濃度為5.0×10,cel
2、ls,mL;②對(duì)照組1,接種單純殘耳軟骨細(xì)胞,細(xì)胞濃度為5.O×107cell“mL;③對(duì)照組2,接種單純ADscs,細(xì)胞濃度為5.O×107ceus,mL;④對(duì)照組3,接種單純殘耳軟骨細(xì)胞,細(xì)胞濃度為2.5×107cells,mL。每組接種6只裸鼠,每只接種0.2mL。體內(nèi)培養(yǎng)10周后取材。通過(guò)對(duì)新生組織的大體觀察、測(cè)量濕重、糖胺多糖含量測(cè)定、組織學(xué)及免疫組化染色等方法對(duì)各組新生軟骨進(jìn)行比較。結(jié)果實(shí)驗(yàn)組、對(duì)照組l與對(duì)照組3產(chǎn)生不同組織量的軟骨樣組織,對(duì)照組2形成纖維樣組織;實(shí)驗(yàn)組平均濕重及糖胺多糖含量達(dá)到對(duì)照組l的88%以上,對(duì)照組3平均濕重低于對(duì)照組l的4
3、0%:HE染色示實(shí)驗(yàn)組、對(duì)照組1與對(duì)照組3的標(biāo)本均有軟骨陷窩形成.對(duì)照組2未見(jiàn)軟骨陷窩形成:Ⅱ型膠原免疫組化染色示實(shí)驗(yàn)組、對(duì)照組l與對(duì)照組3均可見(jiàn)Ⅱ型膠原表達(dá);對(duì)照組2未見(jiàn)Ⅱ型膠原表達(dá)。結(jié)論體內(nèi)共培養(yǎng)條件下,殘耳軟骨微環(huán)境可有效促進(jìn)脂肪干細(xì)胞向軟骨方向分化,殘耳軟骨細(xì)胞與脂肪干細(xì)胞共培養(yǎng)體內(nèi)構(gòu)建軟骨具備可行性?!娟P(guān)鍵詞】共培養(yǎng)脂肪干細(xì)胞殘耳軟骨細(xì)胞體內(nèi)【中圖分類(lèi)號(hào)】Q813.1+2【文獻(xiàn)標(biāo)識(shí)碼】A【文章編號(hào)】1673—0364(2011)02一0075一05TheExpe—m明talstllmesoftheTis踟eEn西眥eringCa州lagebyCo—C
4、IIlt叫ilIgMicrotiaCh叫drocytes鋤dAdipo∞砸豁ue—DeriVedStemCells伽ⅥvDz4ⅣG點(diǎn)e,Jz4ⅣGH咖蛾刪£ere馬Z鼢0y刪夠¨ⅣGQi珧崛刪Ⅳ脅n,SD,vGy印e略忍∞斑
5、su臀¨Ho叩it吐儺i鵬seAco如啊0,脅舭d&婦nce,&可i昭J00J張吼打地corre印D蒯哺眥tb開(kāi)肌^『G日叻伽俾一腫越艮Ji哪曲面∽暾o^醵co州.【Abst憎ct】objec廿veT0explorethefeasibilitvofthechondrogenesisbyco—cuhuringmicmtiachondrocy
6、tesandhumnadiposetissue—derivedstemcells汛鋤o.Me恤0dshADSCsandmicrotiachondrocyteswereisolated流∞i拋.24nudemiceweremndomlydividedinto4groups:①Expgmup'injeetedwithmicmtiachondrocytesandhADSCsbyamixingratioofl:1andtheceUconcentI‘a(chǎn)tionwas5.0×107cells,mL;②Ctrllgroup,injectedwithonlymicrotiac
7、hondmcytes鋤dtheceUconcentrationwas5.o×107cells^llIJ;⑧Ctrl2group,injectedwitlIonlyhADSCsand山eceUconcemrationwas5.0×l護(hù)cell婦k④Ctrl3gmup,injectedwit}lonlymicmtiachondroc”esa11dtIlecellconcentrationw船2.5×107ceUs/mL.6nudemicewereinjectedeachgroupatadose0fo.2mLAnsampJeswerehanrested10weeks
8、aftercuJtu—ng抽鄯西o.Grossobser腿tio玎,averagewetweights,glycos鋤inoglvc獅(GAG)qu蚰tification,histology蚰dimmunohistochemistywereusedtoevaluatethechondrogenesis0faUgmups.ResultsInExp,Ctrl1,andCtd3孕oup,allthespecimensfo冊(cè)edhomogeneouscanilage—liketissuewi出typicalhistolo舀caJstlllctureatdif玷rent
9、extent.InCtll2gmup'