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《egcg對pq誘導(dǎo)的pc12細(xì)胞凋亡的保護(hù)作用及其機制研究》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、第四軍醫(yī)大學(xué)碩士學(xué)位論文學(xué)染色及Westernblotting方法檢測凋亡相關(guān)蛋白(CytoC、Smac)表達(dá)。結(jié)果：Rhodamine123染色顯示，800μmol/LPQ處理24h后，細(xì)胞的熒光強度與對照相比顯著降低(P<0.001)。而EGCG可劑量依賴性的減輕MMP的下降。與PQ組(26.2±2.8%)相比，經(jīng)1、5、10μmol/LEGCG預(yù)處理后，細(xì)胞熒光強度分別增至41.3±3.6%(P<0.01)，56.7±5.4%(P<0.001)，82.9±4.2%(P<0.001)；PQ組與正常對照組相比，Caspase-
2、3活性顯著增強(P<0.001)，而這種激活可被1、5、10μmol/LEGCG預(yù)處理所抑制。與PQ組(216.5±9.6)相比，經(jīng)EGCG預(yù)處理后，Caspase-3活性分別降至179.9±7.6(P<0.001)、123.2±8.1(P<0.001)、68.5±6.1(P<0.001)；免疫細(xì)胞化學(xué)染色顯示正常組細(xì)胞形態(tài)良好，CytoC、Smac染色呈淡棕黃色，均勻分布于胞質(zhì)及突起內(nèi)。PQ處理后部分細(xì)胞變圓，皺縮，CytoC、Smac在細(xì)胞內(nèi)表達(dá)增強，染色呈深棕黃色。相對于PQ組，EGCG預(yù)處理組的CytoC、Smac免疫細(xì)
3、胞化學(xué)染色明顯減弱，細(xì)胞形態(tài)也有改善。Westernblotting檢測Smac蛋白表達(dá)結(jié)果表明PQ處理后胞漿內(nèi)Smac表達(dá)增加，EGCG預(yù)處理可以抑制這種改變。結(jié)論：EGCG對PQ誘導(dǎo)的PC12細(xì)胞凋亡具有保護(hù)作用，機制可能與其維持細(xì)胞MMP、抑制Caspase-3激活、調(diào)節(jié)凋亡相關(guān)蛋白CytoC及Smac在胞漿中的表達(dá)有關(guān)。關(guān)鍵詞:EGCG；百草枯；帕金森??；PC12細(xì)胞；細(xì)胞凋亡；神經(jīng)保護(hù)6第四軍醫(yī)大學(xué)碩士學(xué)位論文NeuroprotectiveeffectsofEGCGonparaquat-inducedapoptosis
4、inPC12cellsanditsmechanismCandidateformaster:HouRongrongSupervisor:ChenJianzongResearchCenterofTraditionalChineseMedicine,Xijinghospital,FourthMilitaryMedicalUniversity,Xi’an710032,ChinaAbstractParkinson'sdisease(PD)isaneurologicaldisordercharacterizedbytheprogressiv
5、edegenerationofdopaminergicneuronsinthesubstantianigraparscompacta.AlthoughtheetiologyofPDremainsunclear,severalriskfactorshavebeenassociatedwiththedisease,includingage,geneticandenvironmentalfactors.Exposuretoagriculturalchemicalsvialivinginaruralenvironment,drinkin
6、gwellwater,oroccupationalexposurehasbeenpostulatedtobeapotentialenvironmentalriskfactor.Recently,awidelyusedherbicidePQhastriggeredpeople’sinterestsbecauseitschemicalstructureisverysimilartoMPP+,theactiveformof1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP).MPTPis
7、aneurotoxinthatisnotnaturallyoccurringbutwhichleadstoparkinsonisminanimalsandhumans.So,thestructuresimilaritybetweenPQandMPP+indicatesthatPQmaybeoneofthepotentialenvironmentalfactorsinPD.EpidemiologicalstudieshavefoundthatthereisanassociationbetweentheuseofPQinagricu
8、ltureandincidenceofPD.Furthermore,previousstudiesindicatethat7第四軍醫(yī)大學(xué)碩士學(xué)位論文PQexposurecausesselectivedegenerationofdopaminergicneuron
