流式常用熒光染料

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1、如何選擇流式細胞儀測定常用的熒光染料流式細胞儀測定常用的熒光染料有很多種,我們?nèi)绾蝸磉x擇流式細胞儀測定的常用熒光染料呢？???首先要考慮，他們分子結(jié)構(gòu)不同,激發(fā)光譜和發(fā)射光譜也不同,選擇熒光染料時，必須依據(jù)流式細胞儀所配備的激光光源的發(fā)射光波長做為重點考慮。發(fā)射光波長，如：氬離子氣體激光管,它的發(fā)射光波488nm；???????????????氦氖離子氣體激光管發(fā)射光波長633nm；????????????????488nm激光光源常用的熒光染料有FITC（異硫氰酸熒光素）；???????????????PE（藻紅蛋白）???????????????PI（碘化丙啶）???

2、?????????????CY5（化青素）???????????????preCP(葉綠素蛋白)???????????????ECD(藻紅蛋白-得克薩斯紅)等。?激發(fā)光和發(fā)射光波長，如下：?激發(fā)光波長（nm）發(fā)射光峰值（nm）FITC488525（綠）PE488575（橙紅）PI488630（橙紅）ECD488610（紅）CY5488675（深紅）PreCP488675（深紅）流式細胞儀中常用的熒光染料光學(xué)特征以及需要的激光器名????稱Ex波長(nm)Em波長(nm)???備????注激光器Hoechst33342355?465?DNA染色核酸標記351nm紫外激光器H

3、oechst33258365?465?DAPI372?456?Indo-1350?405?細胞內(nèi)鈣離子標記FITC490?520?標記抗體488nm?氬?離?子?激?光?器R_PE480?578?PE_CY5480?670?PE_CY5.5480?695?PerCP490?675?AlexaFluor488494?517?PE_TexasRed480?613?Rhodamine123500?540?線粒體標記Fluo-3506?526?細胞內(nèi)鈣離子標記DiOC6(3)480?501?內(nèi)質(zhì)網(wǎng)標記YOYO-1490?510?DNA染色核酸標記PropidiumIodide53

4、0?615?AcridineOrange490?640?APC650?660?標記抗體633nm氦氖激光器AlexaFluor647650?668?CY5650?667?流式?細胞儀交流論壇：http://bbs.bbioo.com/forum-181-1.html熒光染料譜染料名稱Excitation(nm)Emission(nm)分子量備注信息ReactiveandconjugatedprobesHydroxycoumarin325386331SuccinimidylesterAminocoumarin350445330SuccinimidylesterMethoxy

5、coumarin360410317SuccinimidylesterCascadeBlue(375);401423596HydrazidePacificBlue403455406MaleimidePacificOrange403551??Luciferyellow425528??NBD466539294NBD-XR-Phycoerythrin(PE)480;565578240k?PE-Cy5conjugates480;565;650670?akaCychrome,R670,Tri-Color,QuantumRedPE-Cy7conjugates480;565;743767

6、??Red613480;565613?PE-TexasRedPerCP490675?PeridininchlorphyllproteinTruRed490,675695?PerCP-Cy5.5conjugateFluorX494520587GEHealthcareFluorescein495519389FITC;pHsensitiveBODIPY-FL503512??TRITC547572444TRITCX-Rhodamine570576548XRITCLissamineRhodamineB570590??TexasRed589615625Sulfonylchloride

7、Allophycocyanin(APC)650660104k?APC-Cy7conjugates650;755767?PharRedAlexaFluor系列熒光染料AlexaFluor350343442410?AlexaFluor4054014211028?AlexaFluor430434540702?AlexaFluor488499519643QY0.92AlexaFluor500503525700?AlexaFluor514517542714?AlexaFluor532530555724QY0.61AlexaFluor54