t淋巴瘤侵襲轉(zhuǎn)移誘導(dǎo)因子1與胃癌細(xì)胞侵襲移行能力的關(guān)系

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1、T淋巴瘤侵襲轉(zhuǎn)移誘導(dǎo)因子1與胃癌細(xì)胞侵襲移行能力的關(guān)系：朱金明余佩武李翠芳吳淼【摘要】目的檢測胃癌細(xì)胞中T淋巴瘤侵襲轉(zhuǎn)移誘導(dǎo)因子1(Tlymphomainvasionandmetastasisinducingfactor1，Tiam1)的表達(dá)及其與腫瘤侵襲、移行能力的關(guān)系，同時觀察胃癌細(xì)胞在形態(tài)學(xué)方面的變化。方法采用層黏連蛋白黏附法，由胃癌MKN45細(xì)胞株(M0)中篩選獲得高(MH)、低(ML)黏附亞株。應(yīng)用流式細(xì)胞儀檢測Tiam1在胃癌細(xì)胞中的表達(dá)，以Boyden小室法測定M0、ML、MH細(xì)胞的體外侵襲、移行能力，并分析其與Tiam1表達(dá)間的關(guān)系，同時

2、采用蘇木精伊紅及細(xì)胞骨架蛋白染色、掃描電鏡技術(shù)觀察胃癌細(xì)胞形態(tài)。結(jié)果MH細(xì)胞的體外侵襲、移行能力及Tiam1表達(dá)均強(qiáng)于M0、ML細(xì)胞(P<0.05)，且Tiam1表達(dá)與胃癌細(xì)胞體外侵襲、移行能力呈正相關(guān)(r=0.997和1.000)；同時與M0、ML相比，MH胞體伸展，表面突起增多，片狀偽足寬厚，絲狀偽足細(xì)長而密集，異型性明顯，且細(xì)胞骨架結(jié)構(gòu)較紊亂，斑點(diǎn)狀肌動蛋白小體增多。但M0、ML細(xì)胞間差異無統(tǒng)計(jì)學(xué)意義(P>0.05)。結(jié)論Tiam1表達(dá)水平升高可能促進(jìn)胃癌細(xì)胞侵襲、移行。這或許是通過調(diào)整胃癌細(xì)胞骨架結(jié)構(gòu)重組，增強(qiáng)其變形、游走能力而實(shí)現(xiàn)的

3、?！娟P(guān)鍵詞】胃癌；T淋巴瘤侵襲轉(zhuǎn)移誘導(dǎo)因子1；侵襲；移行；形態(tài)學(xué)　　CorrelationbetigrationabilityofgastriccancercellsandTiam1　　【Abstract】ObjectiveToinvestigatetheexpressionofTlymphomainvasionandmetastasisinducingfactor1(Tiam1)ingastriccancercellsanditsrelationshipigrationabilityofgastriccancercells,andobservethemo

4、rphologicalchangesofgastriccancercells.MethodsHighadhesivesubgroups(MH)andloMKN45cellstrain(M0)bylamininadhesionmethodinvitro.TheexpressionsofTiam1inM0,MLandMHcellsetry,theinvasionandmigrationabilityofM0,MLandMHcellsinvitrobyBoydenchamber.ThecorrelationbetigrationabilityofM0,ML,MH

5、cellsandexpressionofTiam1inM0,ML,MHcellsorphologicaldifferencesamongM0,MLandMHcellsatoxylineosinstain,cytoskeletalproteinstainandscanningelectronicmicroscope.ResultsTheinvasionandmigrationabilityandtheexpressionofTiam1inMHcells1igrationabilityofgastriccancercells(r=0.997,1.000).pa

6、redellatepseudopodia,slenderandintensivefilamentouspseudopodia,moreprojectionsonthesurface,derangementofcellskeletonandmoredotlikeactinbodies.There1maypromotetheinvasionandmigrationofgastriccancercellsthroughadjustingthecytoskeletalreconstructionandenhancingthedeformingandmigrati

7、onabilityofgastriccancercells.【Keyphomainvasionandmetastasisinducingfactor1;Invasion;Migration;Morphology腫瘤細(xì)胞的侵襲轉(zhuǎn)移與其運(yùn)動能力密不可分，而有效遷移行為的達(dá)成又于細(xì)胞內(nèi)、外調(diào)節(jié)因子對細(xì)胞骨架動力裝置所賦予的驅(qū)動力以及細(xì)胞骨架成分介導(dǎo)黏附所提供錨定力間的協(xié)調(diào)運(yùn)作。T淋巴瘤侵襲轉(zhuǎn)移誘導(dǎo)因子1(Tlymphomainvasionandmetastasisinducingfactor1，Tiam1)正是作為重要的細(xì)胞骨架結(jié)構(gòu)調(diào)節(jié)因子，而與腫瘤侵襲轉(zhuǎn)移密切

8、相關(guān)［1-3］。本實(shí)驗(yàn)通過檢測Tiam1蛋白在具有不同侵襲、移行能