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1、碩士學(xué)位論文丙戊酸鈉對(duì)胃癌SGC-7901細(xì)胞的放療論文題目增敏作用研究生姓名陳秀萍指導(dǎo)教師姓名楚建軍專(zhuān)業(yè)名稱(chēng)腫瘤學(xué)研究方向腫瘤放療增敏論文提交日期2014年3月2丙戊酸鈉對(duì)胃癌SGC-7901細(xì)胞的放療增敏作用中文摘要中文摘要目的：探討丙戊酸鈉對(duì)胃癌SGC-7901細(xì)胞的放療增敏作用。方法：MTT法檢測(cè)不同劑量及不同時(shí)間丙戊酸鈉對(duì)SGC-7901細(xì)胞的抑制率、用克隆形成實(shí)驗(yàn)檢測(cè)丙戊酸鈉對(duì)SGC-7901細(xì)胞的放療增敏比、流式細(xì)胞術(shù)檢測(cè)細(xì)胞周期差異及細(xì)胞凋亡率。觀察丙戊酸鈉對(duì)胃癌SGC-7901細(xì)胞放療敏感性影響，RT-PCR檢測(cè)各組SGC-7901細(xì)胞HDAC1mRNA及ATMmRNA的
2、表達(dá)情況。結(jié)果：MTT實(shí)驗(yàn)表明丙戊酸鈉對(duì)SGC-7901細(xì)胞作用24h、48h、72h的IC50分別是19.83mmol/L、3.67mmol/L、2.75mmol/L，對(duì)細(xì)胞的抑制具有時(shí)間及劑量的依賴(lài)性；并且對(duì)胃癌細(xì)胞株SGC-7901具有放療增敏作用，放射增敏比為1.31；流式細(xì)胞儀檢測(cè)聯(lián)合組凋亡細(xì)胞數(shù)明顯增加，G0/G1期細(xì)胞比例增高，S期細(xì)胞比例下降，聯(lián)合組與其他實(shí)驗(yàn)組相比差異顯著。丙戊酸鈉聯(lián)合放療能抑制SGC-7901細(xì)胞的HDAC1mRNA及ATMmRNA的表達(dá)（P＜0.05）。結(jié)論：丙戊酸鈉能增強(qiáng)SGC-7901細(xì)胞的放射敏感性，其作用可能是通過(guò)改變細(xì)胞周期再分布、誘導(dǎo)細(xì)胞凋
3、亡及抑制HDAC1mRNA及ATMmRNA表達(dá)等來(lái)實(shí)現(xiàn)的。[關(guān)鍵詞]胃癌；丙戊酸鈉；放療增敏；細(xì)胞周期；細(xì)胞凋亡。作者：陳秀萍指導(dǎo)教師：楚建軍I英文標(biāo)題丙戊酸鈉對(duì)胃癌SGC-7901細(xì)胞的放療增敏作用EnhancementofGastricCarcinomaCellLine
SGC-7901RadiosensitivitybyValproicAcidAbstractObjective:ToinvestigateradiosensitizationeffectofvalproicacidongastriccarcinomacelllineSGC-7901.Methods:MTTassaywa
4、susedtomeasuretheinhibitionrateofgrowthofvalproicacidonongastriccarcinomacellSGC-7901atdifferentconcentrationsanddifferenttimepoint.TheradiosensitizationofvalproicacidonSGC-7901cellswasmeasuredbyclongenicassay.TheapoptosisandcycleofSGC-7901cellsindifferentgroupswereanalyzedbyflowcytometry(FCM).The
5、expressionofHDAC1andATMweredetectedbyRT-PCRineachgroup.Results:Aftertreatedwithvalproicacid,thecellproliferationwasinhibited,cellapoptosiswereincreased,theproportionofSphasewasreducedandtheproportionofG1phasewasincreased.ThecombinationgroupinducedapoptosisonhumangastriccarcinomacellSGC-7901wassign
6、ificantmorethanthealonegroup.Comparedwiththecontrolgroup,cellapoptosisandtheproportionofG0/G1phasewereincreasedsignificantlyinthecombinationgroup,andtheproportionofSphasewasreducedthatassayedbyFCM.TheIC50ofvalproicacidtreatedwithSGC-7901cellsfor24hwas19.83mmol/L,for48hwas3.67mmol/L,andfor72hwas2.7
7、5mmol/L,thecellproliferationwasinhibitedsignificantlyinatime-anddose-dependentmanner.ThevalproicacidhaveradiosensitizationonhumangliomacarcinomacelllineSHG-44,SER=1.31.Theresultoftheflowcytometryshowedthatmoreapo
