復(fù)合應(yīng)用紫杉醇和順鉑對(duì)胃癌細(xì)胞SGC放療的增敏作用.pdf

《復(fù)合應(yīng)用紫杉醇和順鉑對(duì)胃癌細(xì)胞SGC放療的增敏作用.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在應(yīng)用文檔-天天文庫(kù)。

1、·2134·江蘇醫(yī)藥2012年9月第38卷第18期JiangsuMedJ，September2012，Vol38，No．18論著·復(fù)合應(yīng)用紫杉醇和順鉑對(duì)胃癌細(xì)胞SGC放療的增敏作用柏玫蘇筠夏迎秋【摘要】目的觀察紫杉醇、順鉑及兩者聯(lián)合應(yīng)用加放療對(duì)胃癌細(xì)胞株SGC的細(xì)胞增殖與細(xì)胞周期的影響。方法將體外培養(yǎng)的胃癌細(xì)胞株SGC種于96孔板。實(shí)驗(yàn)分為P1組(紫杉醇0．1“g／m1)、P2組(紫杉醇0．01／~g／m1)、C1組(Jf~1鉑o．1ttg／m1)、C2組(順鉑0．O1ttg／m1)、PC1組(紫杉醇0．1~tg／ml+llN鉑0．1ttg／m

2、1)、PC2組(紫杉醇001ttg／ml+llN鉑0．01~g／m1)。無菌環(huán)境下實(shí)施4Gy照射。采用MTT法觀察SGC細(xì)胞增殖抑制率；以流式細(xì)胞技術(shù)檢測(cè)細(xì)胞凋亡及周期變化。結(jié)果PC1組和PC2組胃癌SGC細(xì)胞抑制率分別為(76．6±7．7)和(68．3士2．6)，明顯高于P1組的(46．74-4．5)、P2組的(39．2士5．3)、C1組的(41．4±0．2)和C2組的(25．6±1．3)(P

3、離體胃癌細(xì)胞放療有明顯增敏作用?！娟P(guān)鍵詞】胃癌；SGC細(xì)胞；紫杉醇；順鉑；放療【中圖分類號(hào)】R734【文獻(xiàn)標(biāo)識(shí)碼】A【文章編號(hào)10253-3685(2012)18—2134—03Sensitizingeff~tsofcombineduseofpaclitaxelandcisplatinintensivechemotherapyonradiotherapyingastriccancercellsinvitro，Mei，SUJun，XIAYingqiu．SchoolofPublicHealth，NaingMedicalUniversity，Nanj

4、ing210029，CHINA[Abstract]ObjectiveToobservetheinvitrochangesofcellproliferationandcellcycleofgastriccancereelllineSGCunderwentcombineduseofpaclitaxelandcisplatinintensivechemotherapywithradiotherapy．MethodsTheSGCcellsculturedinvitrowereseededin96一wellplates，whichwereassigned

5、into6groupsofP1(treatedwithpaclitaxel0．1ttg／m1)，P2(treatedwithpaclitaxel0．01／zg／m1)，C1(treatedwithcisplatin0．1ttg／m1)，C2(treatedwithcisplatin0．01／~g／m1)，PC1(P1+C1)andPC2(P2+C2)．The4Gyradiotherapywasperformedundersterilecondition．Thedepressionrateofeellproliferationwascalcula

6、tedwithMTTassay．Thechangesofapoptosisandcellcycleweredetectedbyflowcytometry．ResultsThedepressionratesofcellproliferationingroupsofPC1andPC2were(76．64-7．7)％and(68．3±2．6)，whichwerehigherthan(46．7±4．5)％ingroupPl，(39．24-5．3)ingroupP2(41．4±o．2)ingroupC1，and(25．6±1．3)ingroupC2(P<

7、0．01)．TheSGCcellcyclesaftercombinationofpaclitaxel。cisplatinandradiotherapywereblockedmainlyatstageofG2／M，whichincontrolgroupweremainlyatstageofG1(P

8、forgastriccancercellsinvitro．[Keywords]Gastriccancer；Paclitaxel；Cisplatin；R