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1、萬方數(shù)據(jù)刺參親參促熟原理與健康苗種培育技術(shù)研究KeyTechnologiesandPrinciplesofBroodstockandSeedculturingofSeaCucumber(Apostichopusjaponicus)AbstractTheseacucumber,Apostichopusjaponicus(Selenka)belongstoEchinodermata，Holothuroidea,mainlydistributedinShandong，Liaoning，Hebe．，andthenoahofJiangsuprovinceinChina．Inthela
2、stdecade，seacucumberhasbecomeoneofthemostcommerciallyimportantmariculturespeciesinnorthemChina．Theinnovationalstudiesaboutbroodstocks，larvaeandseedsculturingduringcriticalstagesofAjaponicusarelistedasfollows：Tostudytheeffectsoftemperatureongonadgrowthofadultseacucumber(Apostichopusjaponicu
3、s)duringbreedingseason．，gonaddevelopment,．gametescomposition，foodintake，foodconsumptionrate，oxygenconsumptionrateandsexhormonelevelweredetectedaftertheexperimentalanimalswereculturedatfivetreatments(6，10，14，18and21。C)for80days，andeachtreatmenthadfourreplicates．Wedemonstratedthatgonadindex(
4、GI)，gonadcolor,tubulesdiameter,oocytesdiameterandgametesbiochemicalcompositionvariedsignificantlyatdifferenttemperatures．ThehigherGIandtubulesdiametervalueswererecordedforanimalsheldat14and18。C．however,oocytesdiameterat14。Cwassignificantlylargerthanat18。C．Forfemales，．thelightness∞+)，rednes
5、s@+)andyellowness(6+)valuesofgonadheldatlowertemperatures(10，14and180c)werehigherthan21。C，whileformales，thelowestrednessandyellownessvaluesobtainedat140C．Fattyacidcompositionofmalegonadwasgenerallynotsignificantlyaffectedbytemperature，whileforfemales．，monounsaturatedfattyacidandpolyunsatur
6、atedfattyacidweresignificantlyhigherat140Cthan18and21。C．Inaddition．．thevaluesoffoodintake(6．359／indday)andsexhormonelevel(20．99nmol／LfortheT，390．56pg／mLfortheE2)at14。Caresignificantlyhigherthanothertemperatures，however,thehighestvalueoffoodconsumption(11．79％)isgotat10。C．Theresultsindicated
7、that14'’CIII萬方數(shù)據(jù)刺參親參促熟原理與健康苗種培育技術(shù)研究wastheoptimaltemperatureforgonadgrowthbasedoncomprehensiveanalysesofmorphologicalfeaturesandgonadquality．Incontrast，theanimalsheldatlowertemperatures(6and10。C)andhighertemperature(21。C)couldnotdevelopmenttOmaturationstage．Tos
