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1、(..苧塞笙董塑要..)StudyoftheexpressionofCOX——2ingastriccanceranditsrelationshipwithangiogenesisbyTissueMicroarrayIntroductionGastriccancerisoneofthemalignanttumors,theincidenceandmortalityareincreasing.,111eunlimitedgrowthandmetastasisoftumorcellsisthemostimportantfeature.Itisthemajorreasonforgastric
2、cancerpatients’failuretobecuresanddead.negrowthandmetastasisoftumorsareregulatedbymanyfac-tors.Tissuemicoarray/tissuechipisanewlydevelopedarray—baseshighthroughputtechniquewithaconspicuouscharacteristicofsmallvolumerichininformationthatfacilitatesgeneexpressioninvestigationofverylargenumbersoft
3、umorssimultaneously.Itissuitabletoimmunohistochemistry,DNAorRNAinsitehybridizationwithhishefficiencyandflexibility,anditsresultsarehighlyu-niformwithremarkableCredibilityandcomparability.Manyreportshavedemon-stratedthattissuemicroarray/tissuechipisanewpowerfultechniqueinpatholo-gyoftumorsinvest
4、igation.COX一2isakeyenzymeinPGbiosynthesis.Recently,allenhancedex-pressionofCOX一2hasbeenfoundincasesofcoloncanerandothertumors.andNSAIDscanreducetheriskofcoloncancerinanimalmodels.Manyre.searchsuggestedthatthetulnorgrowthperiodsincludetheangiogenesisperiod.COX-2hastightrelationshipwithgastriccar
5、cinogenesis,buttheactualmecha—nismisnotknown.ObjectiveToexploretheexpressionandclinicalpathologicalsignificanceofCOX一2andMVDimmunohistochemicdllyoncarcinogenesisofgastriccancerbytissue·3·microarraytechnique.Andinvestigatetheirfunctionsininvasionandtherela.tionshipbetweenbiologicalbehaviorsandpr
6、ognosis,expectedtofindmolecularbiologicalmarkerBinpredictingthemetastastiepotentialofgastriccan/2er.Weconstructedtwotissuemicorarrayscontaining225casesofsmall1一咖cylindricalsamplesacquiredfrom104casesofparaffin—embeddedblocksofgastriccancerandnormalgastricmucouswhichderivedfromLiaoningprovinceTu
7、morHospitalandNo.1HospitalofChinaMedicalUniversity。includinggas.triccancerandprecancerouslesionsandnormalgastricmucosa.Detectexpres-sionsofCOX一2andMVDinthetissuemicroarraysofgastriccancerwithEnvi.sion,Theintratumoralmiemvesseldens