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《維甲酸對小鼠b16黑素瘤細胞和正常人黑素細胞的影響》由會員上傳分享，免費在線閱讀，更多相關內(nèi)容在教育資源-天天文庫。
1、醫(yī)學系2000級頒士研究生學位論文ABSTRACTThemelanocyteisthepigment-producingdendr廷icceiloftheepidermis．Mela黔eytesresideinthebasallayerinnormaladultepidermisOnemelanocyteisthereforeincontactwithabout36keratinocytes；togethertheyformtheSO．calledepidermalmelaninunit．Melanocytesaremostcommonlyinepid
2、ermisandfollicle．Melanininvolveseumelaninandpheomelanin，it'SsynthesizedthroughaseriesofstagesinwhichtheenzymetyrosinaseactsonmelaninprecursorstOproducethedenselypigmentedgranules。Tyrosinaseisthekeyenzymeofmelanogenesisanddeterminethesynthesisandquantityofmelanin．Becausemelaninis
3、themainpigmentthatdeterminestheskincolor,someresearcherspaymuchattentiontothemodulationofmetanocytesandmelagenesisTherelationshipbetweenmelanocytesandothercells，thecontactbetweenmelanocytesandendocrinesystemorimmunesystem，theeffectofcytokineandthegenefamilyoftyronasewereinvestig
4、atedbasedOilthecultureofmelanoc”esinvitroThemostpracticalstudyistOfindsomedrugswhicharesafeandeffectivetoregulatethemelanocytesandmelagenesis．Retinoidsareusedabroadnowandwithmuchpharmacologicalactivity．TheeffectofitonskincolorisfoundmclinicWeinvestigatedtheeffectsofretinoicacida
5、ndotherdrugsORB16murinemelanomacellsandnormalhumanmelanocytesinvitro，insearchofthemosteffectiveconcentrationandgetinsightOnthemechanismsinvolved．B16murinemelanomacellsandnormalhumanmelanocyteswerecdturedinvitroThecellsfromthesameoriginalbottleweretransplantedtothe96wellcellcultu
6、reclusterThecellconcentrationwereabout5+105／m1．B16murinemelanomacellswereincubatedfor24hours，whilethenormalhumanmelanocyteswereincubatedfor48hours．Thentheculturemediumwasreplacedandretinoicacidorotherdrugs^醫(yī)學系2000級碩士研究生學位論文wereaddedtoit．Aftertreatingfor72hours，tyrosinaseactivity
7、,melanincontentandeellproliferationrateweredetected．Theresultsshowedthatretinoicacidexhibitedallinhibitoryeffectontyrosinaseactivityandmelaninproductionathighconcentration(>500nmol／L)，andcouldpromotethecellproliferation．Retinoicacidand姆droquinonecouldbecooperativeathigheoncentra
8、tio玨(>500umol／L)．enhancedthedownregulationoftyr
