baff分子在腎移植受者外周血單個(gè)核細(xì)胞的表達(dá)

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1、BAFF分子在腎移植受者外周血單個(gè)核細(xì)胞的表達(dá)中文摘要目的：探討B(tài)AFF分子在腎移植受者術(shù)后不同時(shí)期外周血單個(gè)核細(xì)胞的表達(dá)情況，并初步分析該分子表達(dá)的可能生物學(xué)意義。方法：分別采用免疫熒光標(biāo)記法(IMF)和實(shí)時(shí)定量聚合酶鏈反應(yīng)法(RealTime—PCR)從蛋白水平及RNA水平檢測腎移植受者術(shù)后外周血單個(gè)核細(xì)胞BAFF分子的表達(dá)，實(shí)驗(yàn)分術(shù)后1年以內(nèi)組、術(shù)后1-4年組及術(shù)后5年及以上組，以正常人及血液透析患者作對照。結(jié)果：經(jīng)RealTime—PCR法檢測，術(shù)后1-4年、術(shù)后5年及以上組BAFF分子的表達(dá)均高于健康對照組(P<0．01)，術(shù)后1年以內(nèi)組和健康對照組無明顯差異(P>0．05)

2、。經(jīng)IMF法檢測，BAFF分子在存活5年及以上腎移植受者外周血T淋巴細(xì)胞呈異常高表達(dá)，CD4+T淋巴細(xì)胞和CD8+T淋巴細(xì)胞表面均分布有BAFF分子，CD4+T淋巴細(xì)胞表面BAFF分子的表達(dá)比CD8+T淋巴細(xì)胞高，與對照組比較，均有顯著性差異(P

3、eyTransplantationAbstractObjectivesToinvestigatetheexpressionofBcellactivatingfactorbelongingtot11eTNFfamily(BAFF)inperipheralbloodmononuclearcells(PBMCs)frompatientswithkidneytransplantationandpreliminarydiscussthesignificanceofBAFFonimmunologyofrenaltransplantation．MethodsAccordingtothetimeoft

4、heiroperations，thepatientswereseparatedintothreegroupsjustasfollows：withinayear、amongoneyearandfouryears、afterfiveyears．Moreover,wechoosehealthyvolunteersandhemodialysisascompares．ThelevelofBAFFweremeasuredbvimmunofluorescenceandrealtimempolymerasechainreactioninPBMCsfromthepatientsandcontrolgro

5、ups．ResultsInthecaseoftheexpressionofBAFFithepatientsafterfiveyears，amongoneyearandfouryearshadahigherlevelthanthehealthycontrols(P<0．01)，whilethereisnosignificantdifferenceinothergroupcomparedwiththehealthycontrols．Moreover,BAFFgenerallyexpressesatCD4十a(chǎn)ndCD8+TlymphocyteandtheexpressionofCD4+Tly

6、mphocyteishigherthanCD8十Tlymphocyte．Therehavesignificantdifferencecomparedwiththehealthycontrols(P<0．05)．ConclusionsThesefindingsindicatethatthetrendofBAFF，sexpressiongraduallyincreasefollowingtheincreaseofoperativetime．111epatientsafterfiveyearshaveabnormalhighexpressionintheirTlymphocyte’。。。1‘

7、一．a(chǎn)ndthehighexpresslonmightplayanimportantroleinchronicrejectionafterrenaltransplantation．KeywordsBcellactivatingfactor；Kidneytransplantation；TransplantationimmunologyWrittenbySupervisedbyIlZhongMinHeXiaoZhouBAFF分子在腎移植受者外周血單