資源描述：
《大鼠同種異體骨髓間充質(zhì)干細(xì)胞移植治療急性肝衰竭的實(shí)驗(yàn)的的研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、摘要高其生存率。關(guān)鍵詞：急性肝衰竭；骨髓問充質(zhì)細(xì)胞；實(shí)驗(yàn)研究IIIAbstractABSTRACTObjective：ToevaluatethetherapeuticeffectofBMSCsontheexperimentalacuteliverfailureinRatsinducedbyCCL4．Methods：1．Selecting，separating，cultivatingandidentifyingBMSCswereobtainedfromtworatsthighboneunderasepticcondition，thencu
2、lturedandpuredbythewholemarrow-culturedmethod．Immunohistochemistryisappliedtodetectcell·surfacemarkerexpressions：CD29、CD44、CD34andCD45，whichhelptoidentifyBMSCs．2．EvaluatingthetherapeuticeffectofBMSCsontheexperimentalacuteliverfailureinRatsThirtymodelratswererandomlydivid
3、edintothreegroups：normalcontrolgroup，withoutanytreating；liverfailuregroup，withinjectionofphysiologicalsalineviacaudalvein；transplantationgroup，withBMSCstransplantationofthesamedosage，bothofwhichhadbeeninjectedwith50％CCL4mixedbyparaffinoil，1．2ml／1009，freshly—prepared，tode
4、velopacuteliverfailuremodels．Finally,survivalrates，liverfunctionandhistopathologicexaminationswereperformed，wimtheresultsofwhichtoevaluatethetherapeuticeffectofBMSCstransplantationinthetreatmentofacuteliverfailureinrats．Results：1．1mmunohistochemicalanalysisofP3BMSCsindic
5、atedCD29、CD44positiveandCD34、CD45negative，whichpresentedabrowncellmembraneforpositivemarkers．Flowcytometryshowedpositiveratesrespectively：CD29(99．3％)，CD44(99．6％)，CD34(4．3％)，CD45(17．1％)．2．Ratssurvivalrateshoweditsincreaseto70％，comparedtotheliverfailuregroup(20％)．Besides，t
6、heliverfunctionimprovedgradually,thatis，theactivityofALTandASTdecreasedremarkably．Inpathohistology,thedegenerationandIVAbstractnecrosisofhepaticcellsanddegreeofinflammationwerelessobviousthatofliverfailuregroup．Conclusions：1．BMSCs，withhigh-purityanduniformconfiguration，c
7、ouldbeobtainedbythewhole．marrowadherentculturemethod．’2．BMSCstransplantationviacaudalveiniSaneffectivemethodtotreatacuteliverfailure，increasingitssurvivalrate，improvinghepaticfunctionandalleviatethedegreeofcellnecrosis．Definitely,BMSCstransplantationcallhelptotreatexperi
8、mentalliverfailureinrats．Keywords：Acmeliverfailure；Bonemarrowmesenchymalstemcells；ExperimentalstudyV中英文
