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《中鏈三酰甘油對力竭游泳小鼠抗疲勞的作用研究》由會員上傳分享，免費在線閱讀，更多相關內(nèi)容在學術論文-天天文庫。
1、中鏈三酰甘油對力竭游泳小鼠抗疲勞的作用研究lipidmetabolisminblood，liverandgastrocnemiusmuscle，andreducingtheliveroxidativelevel．Byusingthereal-timequantitativePCRtechnology,theregulativefunctionofMCTforPPAR-aandPGC-laexpressionwasalsofurtherdiscussed．Besides，theexpressionofPPAR-ainliverandAMPK-aingastrocnem
2、iusmusclewereassessedtogetherbyusingtheWesternblottechnology．Inaword，thoseresearchesprovidenewexperimentalevidencesandtheoreticalsupportsforthealleviating-fatigueeffectofMCTinenduranceexercises．Objeetives1．Toaccessthealleviating—fatigueeffectofMCTinenduranceexercises．2．Toresearchthemec
3、hanismofMCTalleviatingphy7siealfatigue．3．ToprovidescientificevidencefortherelationshipbetweenMCTandphysicalfatigue．Methods1．ExperimentalanimalsandGroupsMaleICRmiceinthesestudieswereprovidedbyExperimentalAnimalCenterofSecondMilitaryMedicalUniversity．First，themicewerehousedforanadaptivef
4、eedingoflweekbeforetheexperiment．Second，theywererandomizedintotwelveGroupsbasedontheirbedyweight，with6miceineachgroup．Third，themiceweremarkedfrom1t06iIlonecage．Altogether,therearesixbigGroups(12miceineachgroup)：untreatedcontrolGroups(g1andg12)，untreatedswimminggroup(921and922)，MCT6％gro
5、up(931and932)，MCTl8％group(941and942)，MCT30％group(951and952)，andMCT44．4％group(961and962)．Eachmousewasgiven4．59／dexperimentaldiet，andhadfleeaccesstowater．AttheSalTletime，allGroupsweresubjectedtounloadedswimmingtrainingonMonday,WednesdayandFridayinoneweek．Themiceweretrainedfor20rainwithsu
6、bsequentincreaseof10mineachtimeuntilitreached40min．Theexperimentalperiodslast3weeks．Untreatedcontrolgroup(UCG)anduntreatedswimminggroupfoso)werefedwithD12450Bdiet，withoutcontainingMCT．Theprotein,carbohydrateandfatcontentscorrespondedto20％，70％and10％ofmetabolizableenergy．BothGroupshadswi
7、mmingtrainingduringtheexperimentalperiod．UCGwereconsideredasthebefore-exercisesstatecomparedwithUSGAllMCTGroupsweregiventhemodifiedD12450BdietssupplementeddifferentdosagesofMCT．Amongallthecomponents，lardWassubstitutedinportionbyMCT,whichaccountedfor6％，18％，30％and44．4％ofthetotal缸．AⅡ·5．
