腦康ⅱ號對新生大鼠海馬神經(jīng)干細(xì)胞增殖分化的影響

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1、萬方數(shù)據(jù)±旦±墮墮熊金璺墼盤查!!!!生!旦筮!!鲞蔓!塑堡塾也!里墾叢里叢堡!魚堡：皇￡!!墮蛙!!!!!!!!!：!!!墮!：!·259··論著·腦康Ⅱ號對新生大鼠海馬神經(jīng)干細(xì)胞增殖分化的影響陳玉靜，李林，李宗信，黃小波，陳文強(qiáng)，于曉，王寧群(首都醫(yī)科大學(xué)宣武醫(yī)院，北京100053)【摘要】目的探討腦康I號含藥血清對體外培養(yǎng)大鼠海馬神經(jīng)干細(xì)胞(NSCs)增殖分化作用的影響。方法用無血清DMEM／F12(含20弘g／L堿性成纖維細(xì)胞生長因子(bFGF)和表皮生長因子(EGF)及B27]體外培養(yǎng)新生wistar

2、大鼠海馬NScs，在NSCs分化過程中添加大、中、小劑量(分別為2．o、1．o、o．5kg／L)腦康I號含藥血清進(jìn)行干預(yù)，以免疫熒光及細(xì)胞化學(xué)方法對NSCs及其分化后的細(xì)胞進(jìn)行鑒定。結(jié)果從新生大鼠海馬分離培養(yǎng)的Nscs能夠表達(dá)巢蛋白，并具有分化為神經(jīng)元和星形膠質(zhì)細(xì)胞的能力；5d時，腦康I號各劑量組神經(jīng)球突起長度均顯著長于對照組(P

3、化的神經(jīng)元樣細(xì)胞有促生長、發(fā)育作用。【關(guān)鍵詞】腦康I號，神經(jīng)干細(xì)胞}細(xì)胞培養(yǎng)；細(xì)胞增殖，細(xì)胞分化中圈分類號：R285．5，Q254文獻(xiàn)標(biāo)識碼：ADoI：lO．3969／j．issn．1008—9691．2009．05．001EffectofNaokaⅡgerhao(矗暫康I號)onproHferation觚ddifferentIationofhippocampalneuralstemcellsofm婀饑Imt砒sCHENYU一再'lg，LIL{，l，LIz強(qiáng)g一畦n，HUANGXt∞一bo，CHENW讎一qi勰

4、g，YUXi∞，WANGNing—qmhXt‘‘呲㈣Hos爭it以，C口爭主紐lMedtc口lU越口erst吟，Bet筘拜g100053，Ch

5、眥【Abstr北t】obj佻HveToobservetheeffectofNaokangerhao(NKEH，腦康I號)serumonproliferationanddifferentiationofhippocampalneuralstemcells(NSC8)ofnewbornratsinvitro．MethodsHippocampalNS；CsfromWistar

6、ratsthtwerebornin24hourswereisolatedandcultivatedinv“rotourderzocloneculturing，serumfreeDMEM／F12，20趣／Lbasicfibroblastgrowthfactor(bFGF)，20旭／Lepidermalgrowthfactor(EGF)andB27wereusedintheculture．DuringtheprogressofNSCsdifferentia“on，threedifferentdosesofNKEH(

7、2．O，1．OandO．5kg／L)inserumwereaddedforinterference，andimmunonuorescenceandimmunocytochemistrystainwereappliedtoidentifythecell8intheproliferationanddifferentiation．Resul缸TheneuralspheresobtainedfromculturedhippocampalNSCsofnewbornratswerepositivefornestine】【p

8、ression，withthepotentialforfunhercloningandcapabilityofdifferentiatingintoneuron8orglialcells．0nthe5thdayofcultivation，theneuritelengthofneuralsphereinthethreeNKEHgroupswa8significantlylongerthanthatinthecontrolgroup(P

9、nceshowedthatthepercentagesof}I—tubulinandgliofibrillaryacidprotein(GFAP)p08itiVecellsdifferentiatedfromNSCgindifferentNKEHgroup$weremuchhigherthanthoseincontr01group(P<0．05orP