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《納米雄黃干預(yù)肺癌a549細(xì)胞對(duì)vegf及hif-1表達(dá)的影響》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、提要目的：探討納米雄黃對(duì)人肺腺癌A549細(xì)胞株血管內(nèi)皮生長(zhǎng)因子（VEGF）、缺氧誘導(dǎo)因子（HIF-1）表達(dá)的影響及其抗腫瘤血管生成的機(jī)理。方法：采用細(xì)胞培養(yǎng)方法，運(yùn)用免疫組化法、RT-PCR技術(shù)觀察納米雄黃對(duì)人肺癌A549細(xì)胞VEGF、HIF-1的表達(dá)的影響，進(jìn)而探討納米雄黃抗腫瘤血管生成的作用機(jī)理。結(jié)果：實(shí)驗(yàn)研究證實(shí)，納米雄黃可以抑制人肺癌A549細(xì)胞增殖，隨藥物濃度的增高作用增強(qiáng)，且與順鉑聯(lián)合應(yīng)用有協(xié)同作用，初步揭示納米雄黃抗腫瘤血管生成的機(jī)理可能與下調(diào)VEGF、HIF-1的表達(dá)有關(guān)。結(jié)論：納米雄黃可以通過抑制腫瘤細(xì)胞血管生成等途徑實(shí)現(xiàn)抗腫瘤作用，與順鉑聯(lián)合可產(chǎn)生協(xié)同作
2、用。關(guān)鍵詞納米雄黃；腫瘤血管生成；人肺腺癌A549細(xì)胞；VEGF；HIF-1ExplorerealgarnanoonhumanlungcancerA549cellsVEGF,HIF-1expressionanditsanti-angiogenicmechanismSpeciality:InternalMedicineofTCMAuthor:YuLianyangTutor:ProfessorQiYuanfuAbstractObjective:ToexplorerealgarnanoonhumanlungadenocarcinomaA549celllineofvasculare
3、ndothelialgrowthfactor(VEGF)andhypoxia-induciblefactor(HIF-1)expressionanditsanti-angiogenicmechanism.Methods:Usingcellculturemethods,theuseofimmunohistochemicalmethods,RT-PCRtechniquetoobserverealgarnanoonhumanlungcancerA549cellsofVEGF,HIF-1expression,andthusexplorethenanometerrealgaranti
4、-angiogenicmechanismofaction.Results:TheexperimentalstudyconfirmedthatrealgarnanocaninhibittheproliferationofhumanlungcancerA549cellswithincreaseddrugconcentration,theenhancedroleofcisplatinsynergy,initiallyrevealednanometerrealgaranti-angiogenicmechanismmaybedownwardadjustmentofVEGF,HIF-1
5、expression.Conclusion：Realgarnanothroughtheinhibitionoftumorcellangiogenesispathwaytoachieveanti-tumoreffectofthesynergisticeffectincombinationwithcisplatin.Keywordsnanometerrealgar;tumorangiogenesis;humanlungadenocarcinomaA549cells;VEGF;HIF-1目錄引言...........................................
6、....................................................................................1實(shí)驗(yàn)研究...............................................................................................................................21實(shí)驗(yàn)材料....................................................................
7、.......................................................22細(xì)胞培養(yǎng)...........................................................................................................................33實(shí)驗(yàn)分組......................................................................
