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1、din的破壞，對BBB用的機(jī)制之一。關(guān)鍵詞黃體酮閉鎖小帶蛋白．1咬合蛋白腦缺血再灌注血腦屏障TheFifthClinicalCollegeZhengzhou，Henan，450052，ChinaAbstractBackgroudand0bjectiveincidenceofcerebrovasculardisease(CVD)iSincreasingwithmoreandmoreagingpopulation．CVDhasbecameoneofthethreeleadingcausesofdeathinmankind．Itshighincidence，
2、disabilityandmortalityratesbringheavyeconomicandmentalburdentosocietyandfamily．CVDisdividedintotwotypes：ischemicandhemorrhagic．Ischemiccerebrovasculardiseaseaccountsforabout80％．Currently,theeffectivetreatmentofacuteischemicstrokeisthrombolytictherapyintimewindow．Itbringshopetopa
3、tientswithcerebralinfarction，butalsobringstheproblemofreperfusioninjury．Cerebralischemia—reperfusioninjuryhascomplexpathophysiologicalprocess，andthedestructionofblood-brainbarrierisoneimportantfactor．Blood．brainbarrier(BBB)，whichplaysanimportantroleintheformationofvasogenicbrain
4、edema，isanimportantguaranteetostableofinternalenvironmentofthecentralnervoussystem．ThebasicstructureofBBBincludesbrainmicrovascularendothelialcells(BMECs)andtightjunction(TJ)，basementmembrane，glialmembraneandextracellularmatrixformedbyendfeetofglialcells．TheexpressionofTJprotein
5、sisrelatedcloselytoBBBpermeabilityandbrainedema．ZonulaVandMCA02h／R72hgroups．Progesterone—treatedgroupvehiclegroupweredoneatthepointofthepeakofBBBpermeability．Thereare12ratsineachgroup．Progesterone-treatedgroupvehiclegrouprespectivelyreceivedanintraperitonealinjectionofprogestero
6、ne(8mg／kg)orequalvolumeofvehicleatlhbeforesurgerylh，6hafterreperfusion．2．DetectionofBBBpermeabilityAsamarkerofBBBpermeability，Evansblue(EB)extravasationinthebraintissuewassurveyedbythemeanofspectrophotometrytoevaluatetheBBBpermeability．3．HEstainImmunohistochemistryanalysisHEstai
7、nwasappliedtojudgetheextentofbraindamageischemicregion·SPfstreptavidin．peroxidase)methodwasusedtomeasuretheexpressionVIpermeabilitybegantoincrease，reachedthepeakat24hofreperfusionandwasstillelevatedat72h．Progesteronegroupandvehiclegroupweredoneat24hofreperfusionaftercerebralisch
8、emiafor2h．TheresultshowedthattheamountofEBofpro
